Expresión y cuantificación de RORγt e IL-17 en mucosa intestinal de crías de alpacas (Vicugna pacos)

Translated title of the contribution: Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)

Gustavo A. Murga, Alberto S. Manchego, Gina S. Castro, Danilo C. Pezo, Nieves C. Sandoval

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless sex or breed. Samples were taken from the middle segment of the jejunum. The total RNA was extracted, and the RT-PCR technique was used with specific primers. The relative expression of mRNA of RORγt and IL-17 was determined by the 2-ΔΔCt method using two neonatal pups as calibrators and using GAPDH as an endogenous control. The kinetics of expression of RORγt showed a constant trend during the first and third week of life, reaching its maximum expression in animals from 2 to 7 days of age, unlike IL-17 whose expression was progressive with age, evidencing a high stimulation and expression from the first days of age.

Translated title of the contributionExpression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos)
Original languageSpanish
Pages (from-to)1009-1017
Number of pages9
JournalRevista de Investigaciones Veterinarias del Peru
Volume29
Issue number3
DOIs
StatePublished - 2018

Bibliographical note

Funding Information:
Este trabajo fue financiado por el Pro- grama de Innovación para la Competitividad y Productividad (INNOVATE – Perú) a tra-vés del Proyecto N.° 180 FINCyT-IB-2013.

Publisher Copyright:
© 2018 Universidad Nacional Mayor de San Marcos. All rights reserved.

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