Expression of candidate cold stress and metabolic related genes in Acidithiobacillus ferrivorans PQ33 strain using ferrous iron as electron donor

Gregory Guerra-Bieberach, Robert Ccorahua-Santo, Anika Eca, Jordan Bernaldo, Tito Sánchez, Chachi Rojas-Ayala, Pablo Ramirez

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The identification of genes involved in cold adaptations of psychrotolerant bacteria Acidithiobacillus ferrivorans is important for biomining processes that take place at low temperatures like Andean mining installations in Peru. We have performed relative quantification RT-qPCR on candidate genes to have a role in adaptations at low temperature (5°C). The candidate genes analyzed were six: Two trehalose synthesis pathway genes, trehalose synthase (treS) and malto-oligosiltrehalose trehalohydrolase (treZ) showing no overexpression at 5°C. Two diguanylate cyclases genes related to exopolymer synthesis and biofilm formation (designated as dgc-I and dgc-II in this paper) were overexpressed at 21°C. The rusA and rusB genes involved in iron oxidation showed no significant change for rusA and no expression for rusB gene in any of both conditions. Genes rpoC, gyrB and alaS were validated as reference genes. These results show congruency with trancriptomics studies about gene expression of A. ferrivorans. Furthermore, the trehalose synthesis genes show no overexpression at low temperatures suggesting that other cold adaptation mechanisms are involved.

Original languageEnglish
Title of host publication22nd International Biohydrometallurgy Symposium
EditorsSabrina Hedrich, Axel Schippers, Kathrin Rubberdt, Franz Glombitza, Wolfgang Sand, Wolfgang Sand, Mario Vera Veliz, Sabine Willscher
PublisherTrans Tech Publications Ltd
Pages368-371
Number of pages4
ISBN (Print)9783035711806
DOIs
StatePublished - 2017
Event22nd International Biohydrometallurgy Symposium, 2017 - Freiberg, Germany
Duration: 24 Sep 201727 Sep 2017

Publication series

NameSolid State Phenomena
Volume262 SSP
ISSN (Print)1012-0394
ISSN (Electronic)1662-9779

Conference

Conference22nd International Biohydrometallurgy Symposium, 2017
CountryGermany
CityFreiberg
Period24/09/1727/09/17

Bibliographical note

Funding Information:
This research was financially supported by Innovate Peru (Grant No. 188-FINCYT-IB-2013).

Publisher Copyright:
© 2017 Trans Tech Publications, Switzerland.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • Acidithiobacillus ferrivorans
  • Bioleaching
  • Gene expression
  • Low temperature
  • RT-qPCR

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