Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru

Nisha B. Patel; Raul Y. Tito; Alexandra J. Obregón-Tito; Lindsey O'Neal; Omar Trujillo-Villaroel; Luis Marin-Reyes; Luzmila Troncoso-Corzo; Emilio Guija-Poma; Moriyuki Hamada; Yoshihito Uchino; Cecil M. Lewis; Paul A. Lawson

doi:10.1016/j.anaerobe.2014.12.002

Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru

Nisha B. Patel, Raul Y. Tito, Alexandra J. Obregón-Tito, Lindsey O'Neal, Omar Trujillo-Villaroel, Luis Marin-Reyes, Luzmila Troncoso-Corzo, Emilio Guija-Poma, Moriyuki Hamada, Yoshihito Uchino, Cecil M. Lewis, Paul A. Lawson

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C_16:0 (18.3%), C_18:1ω9c (39.8%), C_18:2ω6,9c/C_{18:0 ANTE} (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2^T (DSM 27367^T=NBRC 109957^T=CCUG 64571^T).

Original language	English
Pages (from-to)	43-48
Number of pages	6
Journal	Anaerobe
Volume	32
DOIs	https://doi.org/10.1016/j.anaerobe.2014.12.002
State	Published - 1 Apr 2015

Bibliographical note

Funding Information:
This study was supported by the U.S. National Institutes of Health ; RO1 GM089886 . We would like to thank Universidad Cientifica del Sur, the personnel at the medical post in Tambo de Mora and the field team of the project Metagenomica e Inclusion Social for sample collection. We also thank Ken-ichiro Suzuki at Biological Resource Center, National Institute of Technology and Evaluation (NBRC) for facilitating the analysis of the peptidoglycan.

Publisher Copyright:
© 2014.

Keywords

16S rRNA-phylogeny
BD
CCUG
Ezakiella peruensis gen. nov., sp. nov.
GPAC
HMP
NBRC
PY
Taxonomy

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10.1016/j.anaerobe.2014.12.002

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Patel, N. B., Tito, R. Y., Obregón-Tito, A. J., O'Neal, L., Trujillo-Villaroel, O., Marin-Reyes, L., Troncoso-Corzo, L., Guija-Poma, E., Hamada, M., Uchino, Y., Lewis, C. M., & Lawson, P. A. (2015). Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru. Anaerobe, 32, 43-48. https://doi.org/10.1016/j.anaerobe.2014.12.002

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title = "Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru",

abstract = "A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T).",

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author = "Patel, {Nisha B.} and Tito, {Raul Y.} and Obreg{\'o}n-Tito, {Alexandra J.} and Lindsey O'Neal and Omar Trujillo-Villaroel and Luis Marin-Reyes and Luzmila Troncoso-Corzo and Emilio Guija-Poma and Moriyuki Hamada and Yoshihito Uchino and Lewis, {Cecil M.} and Lawson, {Paul A.}",

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month = apr,

day = "1",

doi = "10.1016/j.anaerobe.2014.12.002",

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Patel, NB, Tito, RY, Obregón-Tito, AJ, O'Neal, L, Trujillo-Villaroel, O, Marin-Reyes, L, Troncoso-Corzo, L, Guija-Poma, E, Hamada, M, Uchino, Y, Lewis, CM & Lawson, PA 2015, 'Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru', Anaerobe, vol. 32, pp. 43-48. https://doi.org/10.1016/j.anaerobe.2014.12.002

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T1 - Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru

AU - Patel, Nisha B.

AU - Tito, Raul Y.

AU - Obregón-Tito, Alexandra J.

AU - O'Neal, Lindsey

AU - Trujillo-Villaroel, Omar

AU - Marin-Reyes, Luis

AU - Troncoso-Corzo, Luzmila

AU - Guija-Poma, Emilio

AU - Hamada, Moriyuki

AU - Uchino, Yoshihito

AU - Lewis, Cecil M.

AU - Lawson, Paul A.

PY - 2015/4/1

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N2 - A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T).

AB - A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T).

KW - 16S rRNA-phylogeny

KW - BD

KW - CCUG

KW - Ezakiella peruensis gen. nov., sp. nov.

KW - GPAC

KW - HMP

KW - NBRC

KW - PY

KW - Taxonomy

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U2 - 10.1016/j.anaerobe.2014.12.002

DO - 10.1016/j.anaerobe.2014.12.002

M3 - Artículo

C2 - 25481562

AN - SCOPUS:84949117167

SN - 1075-9964

VL - 32

SP - 43

EP - 48

JO - Anaerobe

JF - Anaerobe

ER -

Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru

Abstract

Bibliographical note

Keywords

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