A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T).
Bibliographical noteFunding Information:
This study was supported by the U.S. National Institutes of Health ; RO1 GM089886 . We would like to thank Universidad Cientifica del Sur, the personnel at the medical post in Tambo de Mora and the field team of the project Metagenomica e Inclusion Social for sample collection. We also thank Ken-ichiro Suzuki at Biological Resource Center, National Institute of Technology and Evaluation (NBRC) for facilitating the analysis of the peptidoglycan.
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- 16S rRNA-phylogeny
- Ezakiella peruensis gen. nov., sp. nov.