TY - JOUR
T1 - First report of Streptococcus agalactiae isolated from Oreochromis niloticus in Piura, Peru
T2 - Molecular identification and histopathological lesions
AU - Ortega Asencios, Yessica
AU - Barreiro Sánchez, Frank
AU - Bueno Mendizábal, Hamilton
AU - Huancaré Pusari, Karina
AU - Ostos Alfonso, Henry
AU - Manchego Sayán, Alberto
AU - Pereira Figueiredo, Mayra Araguaia
AU - Gómez Manrique, Wilson
AU - de Andrade Belo, Marco Antonio
AU - Sandoval Chaupe, Nieves
N1 - Publisher Copyright:
© 2016 The Authors
PY - 2016/11/1
Y1 - 2016/11/1
N2 - The aim of this study was to identify the bacterium Streptococcus agalactiae isolated in farmed Nile tilapia (Oreochromis niloticus) from Piura, Peru and to characterize the histopathological lesions caused by this pathogen. Sixteen tilapias were sampled with clinic signs of the disease such as erratic swimming, exophthalmia and haemorrhages on the body and fins. Qualitative PCR in real time and histopathological analysis were performed. Nine fishes positives to S. agalactiae were found. The main histopathological findings were fibrinosuppurative epicarditis, periesplenitis, meninigitis and panophtaltmitis with predominance of mononuclear infiltration in all tissues. The correlation between qPCR and histopathological findings demonstrated nine fish (prevalence of 56.25%) with Cq lower than 30, associated to high degree of tissue injuries. This study reports the first isolation of S. agalactiae by PCR in real time in tilapia farmed in Peru and characterizes the major histopathological changes caused by this bacterium.
AB - The aim of this study was to identify the bacterium Streptococcus agalactiae isolated in farmed Nile tilapia (Oreochromis niloticus) from Piura, Peru and to characterize the histopathological lesions caused by this pathogen. Sixteen tilapias were sampled with clinic signs of the disease such as erratic swimming, exophthalmia and haemorrhages on the body and fins. Qualitative PCR in real time and histopathological analysis were performed. Nine fishes positives to S. agalactiae were found. The main histopathological findings were fibrinosuppurative epicarditis, periesplenitis, meninigitis and panophtaltmitis with predominance of mononuclear infiltration in all tissues. The correlation between qPCR and histopathological findings demonstrated nine fish (prevalence of 56.25%) with Cq lower than 30, associated to high degree of tissue injuries. This study reports the first isolation of S. agalactiae by PCR in real time in tilapia farmed in Peru and characterizes the major histopathological changes caused by this bacterium.
KW - Bacteria
KW - Fish farming
KW - Inflammation
KW - PCR
KW - Tilapia
UR - http://www.scopus.com/inward/record.url?scp=84978115908&partnerID=8YFLogxK
U2 - 10.1016/j.aqrep.2016.06.002
DO - 10.1016/j.aqrep.2016.06.002
M3 - Artículo
AN - SCOPUS:84978115908
SN - 2352-5134
VL - 4
SP - 74
EP - 79
JO - Aquaculture Reports
JF - Aquaculture Reports
ER -