Fraccionamiento y caracterización electroforética de las proteínas de la semilla de kañihua (Chenopodium pallidicaule aellen)

Translated title of the contribution: Fractionation and electrophoretic characterization of (Chenopodium pallidicaule Aellen) kanihua seed proteins

Gladys Moscoso-Mujica, Amparo Zavaleta, Ángel Mujica, Marco Santos, Robert Calixto

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Kanihua (Chenopodium pallidicaule Aellen) is a Chenopodiacea of the andean region, that contains between 15 and 19% protein, with essential amino acids. The objective of the study was to fractionate and electrophoretically characterize the proteins of kanihua seed varieties Cupi-Sayhua and Ramis. In the whole meal, the proximal analysis and fractionation were performed, and the flour was fractionated by five techniques according to Osborne solubility to obtain albumins, globulins, prolamins and glutelins. The methodology, solvents and extraction time were optimized; and the electrophoretic profiles of the fractions were identified. The highest protein content (p≤ 0.05) was of kanihua flour and its protein fractions, compared to kiwicha and wheat. The highest percent yield (p≤ 0.05) during 1 h of sequential extraction of the protein fractions, was obtained with the Rodriguez y et.al., technique for albumins and glutelins, and with the technique described by Barba de la Rosa y et.al., for globulins and prolamins. The following results were found in Ramis and Cupi-Sayhua kanihua: albumins: 15.4±0.3 and 15.8±0.3%, globulins 7S: 24.1±0.5 and 26.3±1.0%, globulins 11S: 25.7±1.0 and 26.7±1.0%, prolamins: 9.6±0.1 and 9.9±0.5% and glutelins: 22.9±0.1 and 21.5±1.4%, respectively. The electrophoretic profile showed patterns similar in number of bands and differences in concentration in both varieties.

Translated title of the contributionFractionation and electrophoretic characterization of (Chenopodium pallidicaule Aellen) kanihua seed proteins
Original languageSpanish
Pages (from-to)144-152
Number of pages9
JournalRevista Chilena de Nutricion
Volume44
Issue number2
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2017, Sociedad Chilena de Nutricion Bromatologia y Toxilogica. All rights reserved.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

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