Further insight into the geographic distribution of leishmania species in Peru by cytochrome b and mannose phosphate isomerase gene analyses

Hirotomo Kato, Abraham G. Cáceres, Chisato Seki, Carmen Rosa Silupu García, Carlos Holguín Mauricci, Salvadora Concepción Castro Martínez, Dafne Moreno Paico, Josefa Leila Castro Muniz, Lucinda Doriz Troyes Rivera, Zoila Isabel Villegas Briones, Silvia Guerrero Quincho, Guísela Lucy Sulca Jayo, Edwin Tineo Villafuerte, Carlos Manrique de Lara Estrada, Fernando Rafael Arias, Fredy Santiago Passara, Nancy Ruelas Llerena, Makoto Kubo, Ahmed Tabbabi, Daisuke S. YamamotoYoshihisa Hashiguchi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


To obtain further insight into geographic distribution of Leishmania species in Peru, a coun-trywide survey, including central to southern rainforest areas where information on causative parasite species is limited, was performed based on cytochrome b (cyt b) and mannose phosphate isomerase (mpi) gene analyses. A total of 262 clinical samples were collected from patients suspected of cutaneous leishmaniasis (CL) in 28 provinces of 13 departments, of which 99 samples were impregnated on FTA (Flinders Technology Associates) cards and 163 samples were Giemsa-stained smears. Leishmania species were successfully identified in 83 (83.8%) of FTA-spotted samples and 59 (36.2%) of Giemsa-stained smear samples. Among the 142 samples identified, the most dominant species was Leishmania (Viannia) braziliensis (47.2%), followed by L. (V.) peruviana (26.1%), and others were L. (V.) guyanensis, L. (V.) lainsoni, L. (V.) shawi, a hybrid of L. (V.) braziliensis and L. (V.) peruviana, and Leishmania (Leishmania) amazonensis. Besides the present epidemiological observations, the current study provided the following findings: 1) A hybrid of L. (V.) braziliensis and L. (V.) peruviana is present outside the Department of Huanuco, the only place reported, 2) Many cases of CL due to L. (V.) lainsoni, an uncommon causative species in Peru, were observed, and 3) L. (V.) shawi is widely circulating in southern Amazonian areas in Peru.

Original languageEnglish
Article numbere0007496
JournalPLoS Neglected Tropical Diseases
Issue number6
StatePublished - Jun 2019

Bibliographical note

Funding Information:
Funding:Thisstudywasfinanciallysupportedby theMinistryofEducation,CultureandSports, ScienceandTechnology(MEXT)ofJapan(Grant Nos.25257501and17H01685).Thefundershad

Funding Information:
This study was financially supported by the Ministry of Education, Culture and Sports, Science and Technology (MEXT) of Japan (Grant Nos. 25257501 and 17H01685). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2019 Kato et al.


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