General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus

F. Costal-Oliveira; C. G. Duarte; R. A. Machado de Avila; M. M. Melo; K. C.F. Bordon; E. C. Arantes; N. C. Paredes; B. Tintaya; C. Bonilla; R. E. Bonilla; W. S. Suarez; A. Yarleque; J. M. Fernandez; E. Kalapothakis; Carlos Chávez-Olórtegui

doi:10.1016/j.toxicon.2012.06.013

General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus

F. Costal-Oliveira, C. G. Duarte, R. A. Machado de Avila, M. M. Melo, K. C.F. Bordon, E. C. Arantes, N. C. Paredes, B. Tintaya, C. Bonilla, R. E. Bonilla, W. S. Suarez, A. Yarleque, J. M. Fernandez, E. Kalapothakis, Carlos Chávez-Olórtegui

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Abstract

This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD₅₀ determined was 0.1 mg/kg and 21.55 mg/kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H. lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old World-scorpion Androctonus australis Hector.

Original language	English
Pages (from-to)	934-942
Number of pages	9
Journal	Toxicon
Volume	60
Issue number	5
DOIs	https://doi.org/10.1016/j.toxicon.2012.06.013
State	Published - Oct 2012

Bibliographical note

Funding Information:
This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – CAPES (Toxinologia No 23038000825/2011-63 ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) and by funds of the INCTTOX Program of Conselho Nacional de Desenvolvimento Científico e Tecnológico , Brazil (CNPq). The authors gratefully acknowledge the support and assistance of the Instituto Nacional de Salud, Peru.

Keywords

Anti-H. lunatus sera
Hadruroides lunatus scorpion venom
LD
Phospholipasic activity

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10.1016/j.toxicon.2012.06.013

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Costal-Oliveira, F., Duarte, C. G., Machado de Avila, R. A., Melo, M. M., Bordon, K. C. F., Arantes, E. C., Paredes, N. C., Tintaya, B., Bonilla, C., Bonilla, R. E., Suarez, W. S., Yarleque, A., Fernandez, J. M., Kalapothakis, E., & Chávez-Olórtegui, C. (2012). General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus. Toxicon, 60(5), 934-942. https://doi.org/10.1016/j.toxicon.2012.06.013

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title = "General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus",

abstract = "This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD50 determined was 0.1 mg/kg and 21.55 mg/kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H. lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old World-scorpion Androctonus australis Hector.",

keywords = "Anti-H. lunatus sera, Hadruroides lunatus scorpion venom, LD, Phospholipasic activity",

author = "F. Costal-Oliveira and Duarte, {C. G.} and {Machado de Avila}, {R. A.} and Melo, {M. M.} and Bordon, {K. C.F.} and Arantes, {E. C.} and Paredes, {N. C.} and B. Tintaya and C. Bonilla and Bonilla, {R. E.} and Suarez, {W. S.} and A. Yarleque and Fernandez, {J. M.} and E. Kalapothakis and Carlos Ch{\'a}vez-Ol{\'o}rtegui",

note = "Funding Information: This research was supported by Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior, Brazil – CAPES (Toxinologia No 23038000825/2011-63 ), Funda{\c c}{\~a}o de Amparo a Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) and by funds of the INCTTOX Program of Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico , Brazil (CNPq). The authors gratefully acknowledge the support and assistance of the Instituto Nacional de Salud, Peru. ",

year = "2012",

month = oct,

doi = "10.1016/j.toxicon.2012.06.013",

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volume = "60",

pages = "934--942",

journal = "Toxicon",

issn = "0041-0101",

publisher = "Elsevier Ltd.",

number = "5",

}

Costal-Oliveira, F, Duarte, CG, Machado de Avila, RA, Melo, MM, Bordon, KCF, Arantes, EC, Paredes, NC, Tintaya, B, Bonilla, C, Bonilla, RE, Suarez, WS, Yarleque, A, Fernandez, JM, Kalapothakis, E & Chávez-Olórtegui, C 2012, 'General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus', Toxicon, vol. 60, no. 5, pp. 934-942. https://doi.org/10.1016/j.toxicon.2012.06.013

TY - JOUR

T1 - General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus

AU - Costal-Oliveira, F.

AU - Duarte, C. G.

AU - Machado de Avila, R. A.

AU - Melo, M. M.

AU - Bordon, K. C.F.

AU - Arantes, E. C.

AU - Paredes, N. C.

AU - Tintaya, B.

AU - Bonilla, C.

AU - Bonilla, R. E.

AU - Suarez, W. S.

AU - Yarleque, A.

AU - Fernandez, J. M.

AU - Kalapothakis, E.

AU - Chávez-Olórtegui, Carlos

N1 - Funding Information: This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – CAPES (Toxinologia No 23038000825/2011-63 ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) and by funds of the INCTTOX Program of Conselho Nacional de Desenvolvimento Científico e Tecnológico , Brazil (CNPq). The authors gratefully acknowledge the support and assistance of the Instituto Nacional de Salud, Peru.

PY - 2012/10

Y1 - 2012/10

N2 - This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD50 determined was 0.1 mg/kg and 21.55 mg/kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H. lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old World-scorpion Androctonus australis Hector.

AB - This communication describes the general biochemical properties and some immunological characteristics of the venom from the Peruvian scorpion Hadruroides lunatus, which is the most medically relevant species in Peru. The soluble venom of this scorpion is toxic to mice, the LD50 determined was 0.1 mg/kg and 21.55 mg/kg when the venom was injected intracranial or intraperitoneally, respectively. The soluble venom displayed proteolytic, hyaluronidasic, phospholipasic and cardiotoxic activities. High performance liquid chromatography of the soluble venom resulted in the separation of 20 fractions. Two peptides with phospholipasic activity were isolated to homogeneity and their molecular masses determined by mass spectrometry (MALDI TOF). Anti-H. lunatus venom sera were produced in rabbits. Western blotting analysis showed that most of the protein content of this venom is immunogenic. H. lunatus anti-venom displayed consistent cross-reactivity with venom antigens from the new World-scorpions Tityus serrulatus and Centruroides sculpturatus venoms; however, a weaker reactivity was observed against the venom antigens from the old World-scorpion Androctonus australis Hector.

KW - Anti-H. lunatus sera

KW - Hadruroides lunatus scorpion venom

KW - LD

KW - Phospholipasic activity

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U2 - 10.1016/j.toxicon.2012.06.013

DO - 10.1016/j.toxicon.2012.06.013

M3 - Artículo

C2 - 22750532

AN - SCOPUS:84864026659

SN - 0041-0101

VL - 60

SP - 934

EP - 942

JO - Toxicon

JF - Toxicon

IS - 5

ER -

General biochemical and immunological characteristics of the venom from Peruvian scorpion Hadruroides lunatus

Abstract

Bibliographical note

Keywords

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