Genetic diversity of the mitochondrial cytochrome b gene in Lutzomyia spp., with special reference to Lutzomyia peruensis, a main vector of Leishmania (Viannia) peruviana in the Peruvian Andes

Kento Yamamoto, Abraham G. Cáceres, Eduardo A. Gomez, Tatsuyuki Mimori, Hiroyuki Iwata, Masataka Korenaga, Tatsuya Sakurai, Ken Katakura, Yoshihisa Hashiguchi, Hirotomo Kato

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The genetic divergence caused by genetic drift and/or selection is suggested to affect the vectorial capacity and insecticide susceptibility of sand flies, as well as other arthropods. In the present study, cytochrome b (cyt b) gene sequences were determined in 13 species circulating in Peru to establish a basis for analysis of the genetic structure, and the intraspecific genetic diversity was assessed in the Lutzomyia (Lu.) peruensis, a main vector species of Leishmania (Viannia) peruviana in Peruvian Andes. Analysis of intraspecific genetic diversity in the cyt b gene sequences from 36 Lu. peruensis identified 3 highly polymorphic sites in the middle region of the gene. Haplotype and gene network analyses were performed on the cyt b gene sequences of 130 Lu. peruensis in 9 Andean areas from 3 Departments (Ancash, Lima and La Libertad). The results showed that the populations of La Libertad were highly polymorphic and that their haplotypes were distinct from those of Ancash and Lima, where dominant haplotypes were observed, suggesting that a population bottleneck may have occurred in Ancash and Lima, but not in La Libertad. The present study indicated that the middle region of the cyt b gene is useful for the analysis of genetic structure in sand fly populations.

Original languageEnglish
Pages (from-to)156-163
Number of pages8
JournalActa Tropica
Volume126
Issue number2
DOIs
StatePublished - May 2013

Bibliographical note

Funding Information:
This study was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan (grant nos. 18256004 and 21780276 ).

Keywords

  • Cytochrome b
  • Haplotype
  • Lutzomyia
  • Sand fly

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