TY - JOUR
T1 - Polimorfismo genético de la apolipoproteína e en una población peruana
AU - Marca, Victoria
AU - Acosta Conchucos, Oscar
AU - Cornejo-Olivas, Mario
AU - Ortega, Olimpio
AU - Huerta Canales De Miranda, Doris Virginia
AU - Mazzetti Soler, Pilar Elena
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - Objectives. To determine the allelic and genotypic frequencies of the APOE gene in a sample of a population group in Peru. Materials and methods. Cross-sectional analytic study in 189 apparently healthy volunteers, workers of the Instituto Nacional de Ciencias Neurológicas in Lima, Perú, divided into 5 groups by birth department and two generations ancestry. Genomic DNA was amplified using PCR-RFLP. The resulting fragments were detected by 12% polyacrylamide gel electrophoresis. Results. The ε3 allele is the most frequent in all the groups (93.9%), with low ε4 (5%) and ε2 (1.1%) allele frequencies. The analysis of heterozygosity (H) for each group displays intermediate diversity between 10 and 20%. Population genetic diversity (Ht) and diversity within populations (Hs) are 14.43% and 14.31% respectively, suggesting genetic proximity between the studied groups for the ApoE polymorphism. Conclusions. Allele frequencies of the ApoE gene found show that allele ε3 has one of the highest frequencies and ε4 allele one of the lowest compared to other population groups in the world, with possible implications in the risk of neurological, cardiovascular and other diseases in our country.
AB - Objectives. To determine the allelic and genotypic frequencies of the APOE gene in a sample of a population group in Peru. Materials and methods. Cross-sectional analytic study in 189 apparently healthy volunteers, workers of the Instituto Nacional de Ciencias Neurológicas in Lima, Perú, divided into 5 groups by birth department and two generations ancestry. Genomic DNA was amplified using PCR-RFLP. The resulting fragments were detected by 12% polyacrylamide gel electrophoresis. Results. The ε3 allele is the most frequent in all the groups (93.9%), with low ε4 (5%) and ε2 (1.1%) allele frequencies. The analysis of heterozygosity (H) for each group displays intermediate diversity between 10 and 20%. Population genetic diversity (Ht) and diversity within populations (Hs) are 14.43% and 14.31% respectively, suggesting genetic proximity between the studied groups for the ApoE polymorphism. Conclusions. Allele frequencies of the ApoE gene found show that allele ε3 has one of the highest frequencies and ε4 allele one of the lowest compared to other population groups in the world, with possible implications in the risk of neurological, cardiovascular and other diseases in our country.
KW - Alleles
KW - Apolipoproteins E
KW - Gene frequency
UR - http://www.scopus.com/inward/record.url?scp=84857091259&partnerID=8YFLogxK
M3 - Artículo
AN - SCOPUS:84857091259
VL - 28
SP - 589
EP - 594
JO - Revista Peruana de Medicina de Experimental y Salud Publica
JF - Revista Peruana de Medicina de Experimental y Salud Publica
SN - 1726-4634
IS - 4
ER -