Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Megumi Fujita; Hirotomo Kato; Abraham G. Cáceres; Eduardo A. Gomez; Lenin Velez; Tatsuyuki Mimori; Feifei Zhang; Hiroyuki Iwata; Masataka Korenaga; Tatsuya Sakurai; Ken Katakura; Yoshihisa Hashiguchi

doi:10.1016/j.actatropica.2011.10.004

Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Megumi Fujita, Hirotomo Kato, Abraham G. Cáceres, Eduardo A. Gomez, Lenin Velez, Tatsuyuki Mimori, Feifei Zhang, Hiroyuki Iwata, Masataka Korenaga, Tatsuya Sakurai, Ken Katakura, Yoshihisa Hashiguchi

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru. © 2011 Elsevier B.V.

Original language	American English
Pages (from-to)	93-98
Number of pages	6
Journal	Acta Tropica
DOIs	https://doi.org/10.1016/j.actatropica.2011.10.004
State	Published - 1 Feb 2012

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10.1016/j.actatropica.2011.10.004

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title = "Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic",

abstract = "Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru. {\textcopyright} 2011 Elsevier B.V.",

author = "Megumi Fujita and Hirotomo Kato and C{\'a}ceres, {Abraham G.} and Gomez, {Eduardo A.} and Lenin Velez and Tatsuyuki Mimori and Feifei Zhang and Hiroyuki Iwata and Masataka Korenaga and Tatsuya Sakurai and Ken Katakura and Yoshihisa Hashiguchi",

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doi = "10.1016/j.actatropica.2011.10.004",

language = "American English",

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T1 - Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

AU - Fujita, Megumi

AU - Kato, Hirotomo

AU - Cáceres, Abraham G.

AU - Gomez, Eduardo A.

AU - Velez, Lenin

AU - Mimori, Tatsuyuki

AU - Zhang, Feifei

AU - Iwata, Hiroyuki

AU - Korenaga, Masataka

AU - Sakurai, Tatsuya

AU - Katakura, Ken

AU - Hashiguchi, Yoshihisa

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru. © 2011 Elsevier B.V.

AB - Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru. © 2011 Elsevier B.V.

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U2 - 10.1016/j.actatropica.2011.10.004

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M3 - Article

SN - 0001-706X

SP - 93

EP - 98

JO - Acta Tropica

JF - Acta Tropica

ER -

Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Abstract

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