Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Megumi Fujita; Hirotomo Kato; Abraham G. Cáceres; Eduardo A. Gomez; Lenin Velez; Tatsuyuki Mimori; Feifei Zhang; Hiroyuki Iwata; Masataka Korenaga; Tatsuya Sakurai; Ken Katakura; Yoshihisa Hashiguchi

doi:10.1016/j.actatropica.2011.10.004

Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Megumi Fujita, Hirotomo Kato, Abraham G. Cáceres, Eduardo A. Gomez, Lenin Velez, Tatsuyuki Mimori, Feifei Zhang, Hiroyuki Iwata, Masataka Korenaga, Tatsuya Sakurai, Ken Katakura, Yoshihisa Hashiguchi

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.

Original language	English
Pages (from-to)	93-98
Number of pages	6
Journal	Acta Tropica
Volume	121
Issue number	2
DOIs	https://doi.org/10.1016/j.actatropica.2011.10.004
State	Published - Feb 2012
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan (Grant Nos. 18256004 and 21780276 ).

Keywords

18S rRNA gene
Genotyping
Lutzomyia
PCR-RFLP
Sand fly

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10.1016/j.actatropica.2011.10.004

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title = "Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic",

abstract = "Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.",

keywords = "18S rRNA gene, Genotyping, Lutzomyia, PCR-RFLP, Sand fly",

author = "Megumi Fujita and Hirotomo Kato and C{\'a}ceres, {Abraham G.} and Gomez, {Eduardo A.} and Lenin Velez and Tatsuyuki Mimori and Feifei Zhang and Hiroyuki Iwata and Masataka Korenaga and Tatsuya Sakurai and Ken Katakura and Yoshihisa Hashiguchi",

note = "Funding Information: This study was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan (Grant Nos. 18256004 and 21780276 ).",

year = "2012",

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doi = "10.1016/j.actatropica.2011.10.004",

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AU - Fujita, Megumi

AU - Kato, Hirotomo

AU - Cáceres, Abraham G.

AU - Gomez, Eduardo A.

AU - Velez, Lenin

AU - Mimori, Tatsuyuki

AU - Zhang, Feifei

AU - Iwata, Hiroyuki

AU - Korenaga, Masataka

AU - Sakurai, Tatsuya

AU - Katakura, Ken

AU - Hashiguchi, Yoshihisa

N1 - Funding Information: This study was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan (Grant Nos. 18256004 and 21780276 ).

PY - 2012/2

Y1 - 2012/2

N2 - Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.

AB - Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.

KW - 18S rRNA gene

KW - Genotyping

KW - Lutzomyia

KW - PCR-RFLP

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DO - 10.1016/j.actatropica.2011.10.004

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AN - SCOPUS:84855967072

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SP - 93

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JO - Acta Tropica

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ER -

Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Abstract

Bibliographical note

Keywords

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