Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Megumi Fujita, Hirotomo Kato, Abraham G. Cáceres, Eduardo A. Gomez, Lenin Velez, Tatsuyuki Mimori, Feifei Zhang, Hiroyuki Iwata, Masataka Korenaga, Tatsuya Sakurai, Ken Katakura, Yoshihisa Hashiguchi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalActa Tropica
Volume121
Issue number2
DOIs
StatePublished - Feb 2012

Bibliographical note

Funding Information:
This study was supported in part by Grants-in-aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan (Grant Nos. 18256004 and 21780276 ).

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

  • 18S rRNA gene
  • Genotyping
  • Lutzomyia
  • PCR-RFLP
  • Sand fly

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