Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa

J. Vásquez, E. A. Florentini, L. A. Camargo, J. Gonzales, M. Valdivia

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15. min exposure time could generate the same results as longer incubation times (30, 45 and 60. min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300. mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37. °C. According to the percentage of reacted spermatozoa, 75-100. mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15. min to be considered sufficient incubation time.

Original languageEnglish
Pages (from-to)618-622
Number of pages5
JournalLivestock Science
Issue number2-3
StatePublished - 2013
Externally publishedYes

Bibliographical note

Funding Information:
This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .


  • Epididymal spermatozoa
  • Hypoosmotic solution
  • Hypoosmotic swelling test
  • Ram


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