Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa

J. Vásquez; E. A. Florentini; L. A. Camargo; J. Gonzales; M. Valdivia

doi:10.1016/j.livsci.2013.08.023

Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa

J. Vásquez, E. A. Florentini, L. A. Camargo, J. Gonzales, M. Valdivia

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15. min exposure time could generate the same results as longer incubation times (30, 45 and 60. min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300. mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37. °C. According to the percentage of reacted spermatozoa, 75-100. mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15. min to be considered sufficient incubation time.

Original language	English
Pages (from-to)	618-622
Number of pages	5
Journal	Livestock Science
Volume	157
Issue number	2-3
DOIs	https://doi.org/10.1016/j.livsci.2013.08.023
State	Published - 2013

Bibliographical note

Funding Information:
This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .

Keywords

Epididymal spermatozoa
Hypoosmotic solution
Hypoosmotic swelling test
Ram

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10.1016/j.livsci.2013.08.023

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title = "Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa",

abstract = "The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15. min exposure time could generate the same results as longer incubation times (30, 45 and 60. min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300. mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37. °C. According to the percentage of reacted spermatozoa, 75-100. mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15. min to be considered sufficient incubation time.",

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author = "J. V{\'a}squez and Florentini, {E. A.} and Camargo, {L. A.} and J. Gonzales and M. Valdivia",

note = "Funding Information: This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .",

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AU - Florentini, E. A.

AU - Camargo, L. A.

AU - Gonzales, J.

AU - Valdivia, M.

N1 - Funding Information: This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .

PY - 2013

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N2 - The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15. min exposure time could generate the same results as longer incubation times (30, 45 and 60. min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300. mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37. °C. According to the percentage of reacted spermatozoa, 75-100. mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15. min to be considered sufficient incubation time.

AB - The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15. min exposure time could generate the same results as longer incubation times (30, 45 and 60. min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300. mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37. °C. According to the percentage of reacted spermatozoa, 75-100. mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15. min to be considered sufficient incubation time.

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KW - Hypoosmotic solution

KW - Hypoosmotic swelling test

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ER -

Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa

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Keywords

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