Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple

Marcelo M. Geraldine; Rosadio A. Raúl; Chero O. Ana; Díaz O. Gerardo; Ciprian C. Aldo; Maturrano H. Lenin

doi:10.15381/rivep.v28i2.13074

Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple

Translated title of the contribution: Identification of salmonella enteritidis and salmonella typhimurium in Guinea pigs by the multiplex PCR

Marcelo M. Geraldine, Rosadio A. Raúl, Chero O. Ana, Díaz O. Gerardo, Ciprian C. Aldo, Maturrano H. Lenin

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from Guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from Guinea pigs.

Translated title of the contribution	Identification of salmonella enteritidis and salmonella typhimurium in Guinea pigs by the multiplex PCR
Original language	Spanish
Pages (from-to)	411-417
Number of pages	7
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	28
Issue number	2
DOIs	https://doi.org/10.15381/rivep.v28i2.13074
State	Published - 1 Apr 2017
Externally published	Yes

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title = "Identificaci{\'o}n de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la T{\'e}cnica de PCR M{\'u}ltiple",

abstract = "The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from Guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from Guinea pigs.",

keywords = "Guinea pigs, Multiplex PCR, Salmonella Enteritidis, Salmonella typhimurium",

author = "Geraldine, {Marcelo M.} and Ra{\'u}l, {Rosadio A.} and Ana, {Chero O.} and Gerardo, {D{\'i}az O.} and Aldo, {Ciprian C.} and Lenin, {Maturrano H.}",

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doi = "10.15381/rivep.v28i2.13074",

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AU - Geraldine, Marcelo M.

AU - Raúl, Rosadio A.

AU - Ana, Chero O.

AU - Gerardo, Díaz O.

AU - Aldo, Ciprian C.

AU - Lenin, Maturrano H.

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AB - The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from Guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from Guinea pigs.

KW - Guinea pigs

KW - Multiplex PCR

KW - Salmonella Enteritidis

KW - Salmonella typhimurium

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SN - 1682-3419

VL - 28

SP - 411

EP - 417

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

IS - 2

ER -

Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple

Abstract

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