Identificación de Yarrowia lipolytica (Ascomycota: Hemiascomycetes) como contaminante en la obtención de amplificados del gen 28S rRNA de moluscos

Jenny Chirinos, Carlos Congrains, Rina Ramírez, Pablo Ramírez

Research output: Contribution to journalArticlepeer-review

Abstract

DNA extractions were performed from foot tissues of land snails using a modified CTAB protocol. PCRs were carried out with universal primers for COI gene and oligonucleotides designed for molluscs, for the markers 16S rRNA, 28S rRNA, and ITS-2. Amplified lengths were 706 pb for COI, 330 pb for 16S rRNA, 900 pb for ITS-2, and 583 pb for 28S rRNA. One amplicon of the last marker was of an unexpected length, ~340 pb. DNA sequences were compared in the GenBank database through the BLASTn program and the sample, with the unexpected length, resulted in 100% identity and 99% query coverage with 26S rRNA gene of the yeast Yarrowia lipolytica. Phylogenetic analysis with Neighbour-Joining and the divergence values confirmed the identification, providing results that support the taxonomic placement of the species within the Hemiascomycetes clade.
Original languageAmerican English
Pages (from-to)59-64
Number of pages6
JournalRevista Peruana de Biologia
Volume17
Issue number1
StatePublished - 1 Apr 2010

Keywords

  • 26S rRNA
  • DNA barcode
  • Fungi
  • Gastropoda

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