Identification of Yarrowia lipolytica (Ascomycota: Hemiascomycetes) as a contaminant in obtaining amplified 28S rRNA gene of mollusks

Translated title of the contribution: Identificación de Yarrowia lipolytica (Ascomycota: Hemiascomycetes) como contaminante en la obtención de amplificados del gen 28S rRNA de moluscos

Jenny Chirinos, Carlos Congrains, Rina Ramírez, Pablo Ramírez

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper we identify an unexpected DNA sequence from the amplicons of 28S rRNA gene of terrestrial mollusks. DNA extractions were performed from foot tissues of land snails using a modified CTAB protocol. PCRs were carried out with universal primers for COI gene and oligonucleotides designed for molluscs, for the markers 16S rRNA, 28S rRNA, and ITS-2. Amplified lengths were 706 pb for COI, 330 pb for 16S rRNA, 900 pb for ITS-2, and 583 pb for 28S rRNA. One amplicon of the last marker was of an unexpected length, ~340 pb. DNA sequences were compared in the GenBank database through the BLASTn program and the sample, with the unexpected length, resulted in 100% identity and 99% query coverage with 26S rRNA gene of the yeast Yarrowia lipolytica. Phylogenetic analysis with Neighbour-Joining and the divergence values confirmed the identification, providing results that support the taxonomic placement of the species within the Hemiascomycetes clade.

Translated title of the contributionIdentificación de Yarrowia lipolytica (Ascomycota: Hemiascomycetes) como contaminante en la obtención de amplificados del gen 28S rRNA de moluscos
Original languageSpanish
Pages (from-to)59-64
Number of pages6
JournalRevista Peruana de Biologia
Volume17
Issue number1
StatePublished - Apr 2010

Bibliographical note

Publisher Copyright:
© Facultad de Ciencias Biológicas UNMSM

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

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