Identificación inmunohistoquímica de las células productoras de somatostatina y polipéptido pancreático en páncreas de alpacas (Vicugna pacos)

Suni P. Nicanor; Navarrete Z. Miluska; Sandoval C. Nieves; Santos R. Francisco; Chávez R. Alexander; Sato S. Alberto

doi:10.15381/rivep.v29i1.14199

Identificación inmunohistoquímica de las células productoras de somatostatina y polipéptido pancreático en páncreas de alpacas (Vicugna pacos)

Translated title of the contribution: Immunohistochemical identification of somatostatin and pancreatic polypeptide producing cells in pancreas of alpacas (Vicugna pacos)

Suni P. Nicanor, Navarrete Z. Miluska, Sandoval C. Nieves, Santos R. Francisco, Chávez R. Alexander, Sato S. Alberto

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The aim of this study was to identify and evaluate gamma (PP) and delta cells in alpaca pancreas by immunohistochemistry (IHC). Samples were obtained from 30 alpacas from the southern highlands of Peru (crías, juveniles and adults). Histological sections of 5 µm thickness (3 per lobe and 2 of the body) were used. A commercial kit of IHC and polyclonal antibodies (anti-PP and anti-SSTR3) were used to identify PP and delta cells. Rat pancreas samples were used as positive controls. The intensity of the marking was qualitatively classified between 0 (no marking) and 3 (strong marking). PP cells were present in all age groups, mainly in the body and right lobe of the pancreas, being observed mainly in the periphery and intermediate zone of the islets with a medium and strong marking. Delta cells were present in both lobes until 4 months of age, without being observed in juveniles or adults. Delta cells were observed mainly on the periphery of the islets with a light marking.

Translated title of the contribution	Immunohistochemical identification of somatostatin and pancreatic polypeptide producing cells in pancreas of alpacas (Vicugna pacos)
Original language	Spanish
Pages (from-to)	95-105
Number of pages	11
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	29
Issue number	1
DOIs	https://doi.org/10.15381/rivep.v29i1.14199
State	Published - 2018

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© 2018 Universidad Nacional Mayor de San Marcos. All Rights Reserved.

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@article{030b11423347449893c78641f4e46998,

title = "Identificaci{\'o}n inmunohistoqu{\'i}mica de las c{\'e}lulas productoras de somatostatina y polip{\'e}ptido pancre{\'a}tico en p{\'a}ncreas de alpacas (Vicugna pacos)",

abstract = "The aim of this study was to identify and evaluate gamma (PP) and delta cells in alpaca pancreas by immunohistochemistry (IHC). Samples were obtained from 30 alpacas from the southern highlands of Peru (cr{\'i}as, juveniles and adults). Histological sections of 5 µm thickness (3 per lobe and 2 of the body) were used. A commercial kit of IHC and polyclonal antibodies (anti-PP and anti-SSTR3) were used to identify PP and delta cells. Rat pancreas samples were used as positive controls. The intensity of the marking was qualitatively classified between 0 (no marking) and 3 (strong marking). PP cells were present in all age groups, mainly in the body and right lobe of the pancreas, being observed mainly in the periphery and intermediate zone of the islets with a medium and strong marking. Delta cells were present in both lobes until 4 months of age, without being observed in juveniles or adults. Delta cells were observed mainly on the periphery of the islets with a light marking.",

keywords = "Alpaca, Immunohisto-chemistry, Pancreas, Pancreatic polypeptide, Somatostatin",

author = "Nicanor, {Suni P.} and Miluska, {Navarrete Z.} and Nieves, {Sandoval C.} and Francisco, {Santos R.} and Alexander, {Ch{\'a}vez R.} and Alberto, {Sato S.}",

year = "2018",

doi = "10.15381/rivep.v29i1.14199",

language = "Espa{\~n}ol",

volume = "29",

pages = "95--105",

journal = "Revista de Investigaciones Veterinarias del Peru",

issn = "1682-3419",

publisher = "Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos",

number = "1",

}

Identificación inmunohistoquímica de las células productoras de somatostatina y polipéptido pancreático en páncreas de alpacas (Vicugna pacos). / Nicanor, Suni P.; Miluska, Navarrete Z.; Nieves, Sandoval C. et al.
In: Revista de Investigaciones Veterinarias del Peru, Vol. 29, No. 1, 2018, p. 95-105.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Identificación inmunohistoquímica de las células productoras de somatostatina y polipéptido pancreático en páncreas de alpacas (Vicugna pacos)

AU - Nicanor, Suni P.

AU - Miluska, Navarrete Z.

AU - Nieves, Sandoval C.

AU - Francisco, Santos R.

AU - Alexander, Chávez R.

AU - Alberto, Sato S.

PY - 2018

Y1 - 2018

N2 - The aim of this study was to identify and evaluate gamma (PP) and delta cells in alpaca pancreas by immunohistochemistry (IHC). Samples were obtained from 30 alpacas from the southern highlands of Peru (crías, juveniles and adults). Histological sections of 5 µm thickness (3 per lobe and 2 of the body) were used. A commercial kit of IHC and polyclonal antibodies (anti-PP and anti-SSTR3) were used to identify PP and delta cells. Rat pancreas samples were used as positive controls. The intensity of the marking was qualitatively classified between 0 (no marking) and 3 (strong marking). PP cells were present in all age groups, mainly in the body and right lobe of the pancreas, being observed mainly in the periphery and intermediate zone of the islets with a medium and strong marking. Delta cells were present in both lobes until 4 months of age, without being observed in juveniles or adults. Delta cells were observed mainly on the periphery of the islets with a light marking.

AB - The aim of this study was to identify and evaluate gamma (PP) and delta cells in alpaca pancreas by immunohistochemistry (IHC). Samples were obtained from 30 alpacas from the southern highlands of Peru (crías, juveniles and adults). Histological sections of 5 µm thickness (3 per lobe and 2 of the body) were used. A commercial kit of IHC and polyclonal antibodies (anti-PP and anti-SSTR3) were used to identify PP and delta cells. Rat pancreas samples were used as positive controls. The intensity of the marking was qualitatively classified between 0 (no marking) and 3 (strong marking). PP cells were present in all age groups, mainly in the body and right lobe of the pancreas, being observed mainly in the periphery and intermediate zone of the islets with a medium and strong marking. Delta cells were present in both lobes until 4 months of age, without being observed in juveniles or adults. Delta cells were observed mainly on the periphery of the islets with a light marking.

KW - Alpaca

KW - Immunohisto-chemistry

KW - Pancreas

KW - Pancreatic polypeptide

KW - Somatostatin

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U2 - 10.15381/rivep.v29i1.14199

DO - 10.15381/rivep.v29i1.14199

M3 - Artículo

AN - SCOPUS:85045930041

SN - 1682-3419

VL - 29

SP - 95

EP - 105

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

IS - 1

ER -

Identificación inmunohistoquímica de las células productoras de somatostatina y polipéptido pancreático en páncreas de alpacas (Vicugna pacos)

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