Identificación molecular de salmonella typhimurium en cuyes al primer parto mediante la técnica de PCR multiple

Chero O. Ana; Rosadio A. Raúl; Marcelo M. Geraldine; Díaz O. Gerardo; Jiménez A. Ronald; Castro G. Yenny; Maturrano H. Lenin

doi:10.15381/rivep.v28.i3.13288

Identificación molecular de salmonella typhimurium en cuyes al primer parto mediante la técnica de PCR multiple

Translated title of the contribution: Molecular identification of salmonella typhimurium and enteritidis in Guinea pigs at first parturition by multiplex PCR

Chero O. Ana, Rosadio A. Raúl, Marcelo M. Geraldine, Díaz O. Gerardo, Jiménez A. Ronald, Castro G. Yenny, Maturrano H. Lenin

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

Translated title of the contribution	Molecular identification of salmonella typhimurium and enteritidis in Guinea pigs at first parturition by multiplex PCR
Original language	Spanish
Pages (from-to)	679-686
Number of pages	8
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	28
Issue number	3
DOIs	https://doi.org/10.15381/rivep.v28.i3.13288
State	Published - 1 Jul 2017
Externally published	Yes

Bibliographical note

Funding Information:
Los autores expresan su agradecimien- to al Programa Nacional de Innovación para la CompetitividadyProductividad –Innóvate Perú, fuente financiadora del Proyecto «De-sarrollo de una vacuna para el control y pre-vención de la salmonelosis en la producción de cuyes», Contrato N° 362-PNICP-PIAP-2014.Alos propietarios del centro de crianza de cuyes, Sres. Eduardo López yYenny Castro, por brindar lasfacilidades para realizar el estudio. A Laura Córdova, Gerardo Díaz y Geraldine Marcelo por su apoyo incondicio-nal durante las tomas de muestra y a Rocío

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10.15381/rivep.v28.i3.13288

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title = "Identificaci{\'o}n molecular de salmonella typhimurium en cuyes al primer parto mediante la t{\'e}cnica de PCR multiple",

abstract = "The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.",

keywords = "Females at first parturition, Guinea pigs, Multiplex PCR, Salmonella",

author = "Ana, {Chero O.} and Ra{\'u}l, {Rosadio A.} and Geraldine, {Marcelo M.} and Gerardo, {D{\'i}az O.} and Ronald, {Jim{\'e}nez A.} and Yenny, {Castro G.} and Lenin, {Maturrano H.}",

note = "Funding Information: Los autores expresan su agradecimien- to al Programa Nacional de Innovaci{\'o}n para la CompetitividadyProductividad –Inn{\'o}vate Per{\'u}, fuente financiadora del Proyecto «De-sarrollo de una vacuna para el control y pre-venci{\'o}n de la salmonelosis en la producci{\'o}n de cuyes», Contrato N° 362-PNICP-PIAP-2014.Alos propietarios del centro de crianza de cuyes, Sres. Eduardo L{\'o}pez yYenny Castro, por brindar lasfacilidades para realizar el estudio. A Laura C{\'o}rdova, Gerardo D{\'i}az y Geraldine Marcelo por su apoyo incondicio-nal durante las tomas de muestra y a Roc{\'i}o",

year = "2017",

month = jul,

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doi = "10.15381/rivep.v28.i3.13288",

language = "Espa{\~n}ol",

volume = "28",

pages = "679--686",

journal = "Revista de Investigaciones Veterinarias del Peru",

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publisher = "Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos",

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T1 - Identificación molecular de salmonella typhimurium en cuyes al primer parto mediante la técnica de PCR multiple

AU - Ana, Chero O.

AU - Raúl, Rosadio A.

AU - Geraldine, Marcelo M.

AU - Gerardo, Díaz O.

AU - Ronald, Jiménez A.

AU - Yenny, Castro G.

AU - Lenin, Maturrano H.

N1 - Funding Information: Los autores expresan su agradecimien- to al Programa Nacional de Innovación para la CompetitividadyProductividad –Innóvate Perú, fuente financiadora del Proyecto «De-sarrollo de una vacuna para el control y pre-vención de la salmonelosis en la producción de cuyes», Contrato N° 362-PNICP-PIAP-2014.Alos propietarios del centro de crianza de cuyes, Sres. Eduardo López yYenny Castro, por brindar lasfacilidades para realizar el estudio. A Laura Córdova, Gerardo Díaz y Geraldine Marcelo por su apoyo incondicio-nal durante las tomas de muestra y a Rocío

PY - 2017/7/1

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N2 - The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

AB - The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

KW - Females at first parturition

KW - Guinea pigs

KW - Multiplex PCR

KW - Salmonella

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U2 - 10.15381/rivep.v28.i3.13288

DO - 10.15381/rivep.v28.i3.13288

M3 - Artículo

AN - SCOPUS:85040343970

SN - 1682-3419

VL - 28

SP - 679

EP - 686

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

IS - 3

ER -

Identificación molecular de salmonella typhimurium en cuyes al primer parto mediante la técnica de PCR multiple

Abstract

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