Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

O. Ana Chero; A. Raúl Rosadio; M. Geraldine Marcelo; O. Gerardo Díaz; A. Ronald Jiménez; G. Yenny Castro; H. Lenin Maturrano

doi:10.15381/RIVEP.V28I3.13288

Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

Translated title of the contribution: Molecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR

O. Ana Chero, A. Raúl Rosadio, M. Geraldine Marcelo, O. Gerardo Díaz, A. Ronald Jiménez, G. Yenny Castro, H. Lenin Maturrano

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

Translated title of the contribution	Molecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR
Original language	Spanish
Pages (from-to)	679-686
Number of pages	8
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	28
Issue number	3
DOIs	https://doi.org/10.15381/RIVEP.V28I3.13288
State	Published - 11 Oct 2017

Bibliographical note

Funding Information:
Los autores expresan su agradecimiento al Programa Nacional de Innovación para la Competitividad y Productividad - Innóvate Perú, fuente financiadora del Proyecto «Desarrollo de una vacuna para el control y prevención de la salmonelosis en la producción de cuyes», Contrato N° 362-PNICP-PIAP-2014. A los propietarios del centro de crianza de cuyes, Sres. Eduardo López y Yenny Castro, por brindar las facilidades para realizar el estudio. A Laura Córdova, Gerardo Díaz y Geraldine Marcelo por su apoyo incondicional durante las tomas de muestra y a Rocío Rimac por su aporte en el desarrollo del estudio.

Publisher Copyright:
© 2017 Universidad Nacional Mayor de San Marcos. All rights reserved.

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title = "Identificaci{\'o}n molecular de Salmonella Typhimurium en cuyes al primer parto mediante la t{\'e}cnica de PCR m{\'u}ltiple",

abstract = "The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.",

keywords = "Females at first parturition, Guinea pigs, Multiplex PCR, Salmonella",

author = "{Ana Chero}, O. and {Ra{\'u}l Rosadio}, A. and {Geraldine Marcelo}, M. and {Gerardo D{\'i}az}, O. and {Ronald Jim{\'e}nez}, A. and {Yenny Castro}, G. and {Lenin Maturrano}, H.",

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doi = "10.15381/RIVEP.V28I3.13288",

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Ana Chero, O, Raúl Rosadio, A, Geraldine Marcelo, M, Gerardo Díaz, O, Ronald Jiménez, A, Yenny Castro, G & Lenin Maturrano, H 2017, 'Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple', Revista de Investigaciones Veterinarias del Peru, vol. 28, no. 3, pp. 679-686. https://doi.org/10.15381/RIVEP.V28I3.13288

TY - JOUR

T1 - Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

AU - Ana Chero, O.

AU - Raúl Rosadio, A.

AU - Geraldine Marcelo, M.

AU - Gerardo Díaz, O.

AU - Ronald Jiménez, A.

AU - Yenny Castro, G.

AU - Lenin Maturrano, H.

PY - 2017/10/11

Y1 - 2017/10/11

N2 - The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

AB - The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

KW - Females at first parturition

KW - Guinea pigs

KW - Multiplex PCR

KW - Salmonella

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M3 - Artículo

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SN - 1682-3419

VL - 28

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EP - 686

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

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ER -

Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

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