Abstract
The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.
Translated title of the contribution | Molecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR |
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Original language | Spanish |
Pages (from-to) | 679-686 |
Number of pages | 8 |
Journal | Revista de Investigaciones Veterinarias del Peru |
Volume | 28 |
Issue number | 3 |
DOIs | |
State | Published - 11 Oct 2017 |
Bibliographical note
Funding Information:Los autores expresan su agradecimiento al Programa Nacional de Innovación para la Competitividad y Productividad - Innóvate Perú, fuente financiadora del Proyecto «Desarrollo de una vacuna para el control y prevención de la salmonelosis en la producción de cuyes», Contrato N° 362-PNICP-PIAP-2014. A los propietarios del centro de crianza de cuyes, Sres. Eduardo López y Yenny Castro, por brindar las facilidades para realizar el estudio. A Laura Córdova, Gerardo Díaz y Geraldine Marcelo por su apoyo incondicional durante las tomas de muestra y a Rocío Rimac por su aporte en el desarrollo del estudio.
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