Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

O. Ana Chero; A. Raúl Rosadio; M. Geraldine Marcelo; O. Gerardo Díaz; A. Ronald Jiménez; G. Yenny Castro; H. Lenin Maturrano

doi:10.15381/RIVEP.V28I3.13288

Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

Translated title of the contribution: Molecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR

O. Ana Chero, A. Raúl Rosadio, M. Geraldine Marcelo, O. Gerardo Díaz, A. Ronald Jiménez, G. Yenny Castro, H. Lenin Maturrano

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

Translated title of the contribution	Molecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR
Original language	Spanish
Pages (from-to)	679-686
Number of pages	8
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	28
Issue number	3
DOIs	https://doi.org/10.15381/RIVEP.V28I3.13288
State	Published - 11 Oct 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Universidad Nacional Mayor de San Marcos. All rights reserved.

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title = "Identificaci{\'o}n molecular de Salmonella Typhimurium en cuyes al primer parto mediante la t{\'e}cnica de PCR m{\'u}ltiple",

abstract = "The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.",

keywords = "Females at first parturition, Guinea pigs, Multiplex PCR, Salmonella",

author = "{Ana Chero}, O. and {Ra{\'u}l Rosadio}, A. and {Geraldine Marcelo}, M. and {Gerardo D{\'i}az}, O. and {Ronald Jim{\'e}nez}, A. and {Yenny Castro}, G. and {Lenin Maturrano}, H.",

year = "2017",

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doi = "10.15381/RIVEP.V28I3.13288",

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journal = "Revista de Investigaciones Veterinarias del Peru",

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publisher = "Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos",

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Ana Chero, O, Raúl Rosadio, A, Geraldine Marcelo, M, Gerardo Díaz, O, Ronald Jiménez, A, Yenny Castro, G & Lenin Maturrano, H 2017, 'Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple', Revista de Investigaciones Veterinarias del Peru, vol. 28, no. 3, pp. 679-686. https://doi.org/10.15381/RIVEP.V28I3.13288

TY - JOUR

T1 - Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

AU - Ana Chero, O.

AU - Raúl Rosadio, A.

AU - Geraldine Marcelo, M.

AU - Gerardo Díaz, O.

AU - Ronald Jiménez, A.

AU - Yenny Castro, G.

AU - Lenin Maturrano, H.

PY - 2017/10/11

Y1 - 2017/10/11

N2 - The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

AB - The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

KW - Females at first parturition

KW - Guinea pigs

KW - Multiplex PCR

KW - Salmonella

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U2 - 10.15381/RIVEP.V28I3.13288

DO - 10.15381/RIVEP.V28I3.13288

M3 - Artículo

AN - SCOPUS:85119499578

SN - 1682-3419

VL - 28

SP - 679

EP - 686

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

IS - 3

ER -

Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

Abstract

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