TY - JOUR
T1 - Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP
AU - Koarashi, Yu
AU - Cáceres, Abraham G.
AU - Saca, Florencia Margarita Zúniga
AU - Flores, Elsa Elvira Palacios
AU - Trujillo, Adela Celis
AU - Alvares, José Luis Abanto
AU - Yoshimatsu, Kumiko
AU - Arikawa, Jiro
AU - Katakura, Ken
AU - Hashiguchi, Yoshihisa
AU - Kato, Hirotomo
PY - 2016/6/1
Y1 - 2016/6/1
N2 - © 2016 Elsevier B.V. A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.
AB - © 2016 Elsevier B.V. A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84959265318&origin=inward
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84959265318&origin=inward
U2 - 10.1016/j.actatropica.2016.02.024
DO - 10.1016/j.actatropica.2016.02.024
M3 - Article
SN - 0001-706X
SP - 83
EP - 87
JO - Acta Tropica
JF - Acta Tropica
ER -