Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP

Yu Koarashi; Abraham G. Cáceres; Florencia Margarita Zúniga Saca; Elsa Elvira Palacios Flores; Adela Celis Trujillo; José Luis Abanto Alvares; Kumiko Yoshimatsu; Jiro Arikawa; Ken Katakura; Yoshihisa Hashiguchi; Hirotomo Kato

doi:10.1016/j.actatropica.2016.02.024

Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP

Yu Koarashi, Abraham G. Cáceres, Florencia Margarita Zúniga Saca, Elsa Elvira Palacios Flores, Adela Celis Trujillo, José Luis Abanto Alvares, Kumiko Yoshimatsu, Jiro Arikawa, Ken Katakura, Yoshihisa Hashiguchi, Hirotomo Kato

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19 Scopus citations

Abstract

A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.

Original language	English
Pages (from-to)	83-87
Number of pages	5
Journal	Acta Tropica
Volume	158
DOIs	https://doi.org/10.1016/j.actatropica.2016.02.024
State	Published - 1 Jun 2016

Bibliographical note

Funding Information:
This study was financially supported by the Ministry of Education, Culture and Sports, Science and Technology (MEXT) of Japan (Grant Nos. 23256002 and 25257501).

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

Leishmania
Mannose phosphate isomerase
PCR-RFLP
Peru

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10.1016/j.actatropica.2016.02.024

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Koarashi, Y., Cáceres, A. G., Saca, F. M. Z., Flores, E. E. P., Trujillo, A. C., Alvares, J. L. A., Yoshimatsu, K., Arikawa, J., Katakura, K., Hashiguchi, Y., & Kato, H. (2016). Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP. Acta Tropica, 158, 83-87. https://doi.org/10.1016/j.actatropica.2016.02.024

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title = "Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP",

abstract = "A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.",

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Koarashi, Y, Cáceres, AG, Saca, FMZ, Flores, EEP, Trujillo, AC, Alvares, JLA, Yoshimatsu, K, Arikawa, J, Katakura, K, Hashiguchi, Y & Kato, H 2016, 'Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP', Acta Tropica, vol. 158, pp. 83-87. https://doi.org/10.1016/j.actatropica.2016.02.024

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AU - Koarashi, Yu

AU - Cáceres, Abraham G.

AU - Saca, Florencia Margarita Zúniga

AU - Flores, Elsa Elvira Palacios

AU - Trujillo, Adela Celis

AU - Alvares, José Luis Abanto

AU - Yoshimatsu, Kumiko

AU - Arikawa, Jiro

AU - Katakura, Ken

AU - Hashiguchi, Yoshihisa

AU - Kato, Hirotomo

PY - 2016/6/1

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N2 - A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.

AB - A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.

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ER -

Identification of causative leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP

Abstract

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Keywords

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