Identificación de las células de paneth en el intestino delgado de alpacas en los primeros 21 días de edad

Translated title of the contribution: Identification of paneth cells in the small intestine of alpacas in the first 21 days of age

Boris Antonio Lira Mejia, M. Sergio Cueva, Jose Luis Rodriguez Gutierrez, S. Milder Ayón, Carolina Zanuzzi, Claudio Barbeito, P. Néstor Falcón, Maria Elith Vasquez Cachay

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Paneth cells have an important function in the defense and protection mechanisms of the gastrointestinal tract in several animal species through secretions, e.g. as lysozime, secretory phospholipase A2 and defensins. The aim of this study was to identify the Paneth cells in the small intestine of baby alpacas. Eighteen culled animals between 1 and 21 days of age were used. Duodenum, jejunum and ileum samples were taken. Samples were fixed in 10% formalin, processed and stained with haematoxylin-eosin (H-E) and Masson trichromic. An anti-lizozime polyclonal antibody was used for immunohistochemistry. Typical Paneth cells were not identified with H-E and Masson trichromic stains, whereas lysozime granules were identified in the base of the crypts of Lieberkühn of duodenum, jejunum and ileum by immunohistochemistry since the first day of age. The number in jejunum and ileum was greater between 15 and 21 days of age. The cell area was between 129.19 and 147.67μm2, major axes between 17.96 and 19.92 μm and minor axes between 8.68 and 9.79 μm. It is concluded that the observed cells corresponded to Paneth cells.

Translated title of the contributionIdentification of paneth cells in the small intestine of alpacas in the first 21 days of age
Original languageSpanish
Pages (from-to)138-146
Number of pages9
JournalRevista de Investigaciones Veterinarias del Peru
Volume23
Issue number2
StatePublished - 2012
Externally publishedYes

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