Identification of persistently infected cattle and genotype of bovine viral diarrhea virus in cattle of Anta, Cusco, Peru

G. Edgar Valdez, P. Ignasio Pacheco, A. Walter Vergara, L. Juan Pinto, B. Fiorela Fernández, F. Fiorela Guzmán, M. Dennis Navarro, G. Hermelinda Rivera

Research output: Contribution to journalArticle

Abstract

© 2018 Universidad Nacional Mayor de San Marcos. All Rights Reserved. The aim of this study was to identify persistently infected cattle (PI) and to identify the viral diarrhea virus genotype (BVDV) in cattle of small farmers in five districts of the province of Anta, Cusco, which were negative for antibodies against BVDV (n=558) in a previous study. The identification of the PI animals was carried out in the 558 serum samples of female bovine Holstein, Brown Swiss and Creoles of various ages, by the capture ELISA test. The identification of the viral genotype was carried out in four samples of PI cattle through the RT-PCR real-time. The results showed that 7.2% (40/558) of the cattle were positive for viral antigen in a first analysis, where the antigen was detected in animals older than 6 months up to 5 years in the three breeds. In the second analysis carried out 30 day after in the 40 positives, 30% (12/40) continued to be positive for viral antigen indicating that they were PI animals between 6 months and 4 years in four of the five Anta districts. The prevalence of PI animals in the bovine population sampled in province of Anta was 2.2% (12/558). Real-time RT-PCR showed the amplification of a specific product corresponding to genotype 1 (VDVB-1). There was no amplification of the target sequence of genotype 2.
Original languageAmerican English
Pages (from-to)1527-1537
Number of pages11
JournalRevista de Investigaciones Veterinarias del Peru
DOIs
StatePublished - 1 Jan 2018

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    Edgar Valdez, G., Ignasio Pacheco, P., Walter Vergara, A., Juan Pinto, L., Fiorela Fernández, B., Fiorela Guzmán, F., Dennis Navarro, M., & Hermelinda Rivera, G. (2018). Identification of persistently infected cattle and genotype of bovine viral diarrhea virus in cattle of Anta, Cusco, Peru. Revista de Investigaciones Veterinarias del Peru, 1527-1537. https://doi.org/10.15381/rivep.v29i4.15192