TY - JOUR
T1 - Identification of proteins associated with the formation of oral biofilms
AU - Millones-Gómez, Pablo Alejandro
AU - Amaranto, Reyma Evelyn Bacilio
AU - Torres, Dora Jesús Maurtua
AU - Calla-Poma, Roger Damaso
AU - Requena-Mendizabal, Margarita F.
AU - Alvino-Vales, María Isabel
AU - Calla-Poma, Rubén
N1 - Publisher Copyright:
© 2021, Association of Support to Oral Health Research (APESB). All rights reserved.
PY - 2021
Y1 - 2021
N2 - Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.
AB - Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.
KW - Dental Plaque
KW - Fusobacterium nucleatum
KW - Genomics
KW - Streptococcus gordonii
UR - http://www.scopus.com/inward/record.url?scp=85105451126&partnerID=8YFLogxK
U2 - 10.1590/pboci.2021.084
DO - 10.1590/pboci.2021.084
M3 - Artículo
AN - SCOPUS:85105451126
SN - 1519-0501
VL - 21
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
M1 - e0128
ER -