Identification, Quantification, and Characterization of the Phenolic Fraction of Brunfelsia grandiflora: In Vitro Antioxidant Capacity

Raquel Mateos, Norma Ramos-Cevallos, Americo Castro-Luna, Mariella Ramos Gonzalez, Zoyla Mirella Clavo, Miguel Quispe-Solano, Luis Goya, Jose Luis Rodriguez Gutierrez

Research output: Contribution to journalArticlepeer-review


Brunfelsia grandiflora is an ancient plant widely used for its promising medicinal properties, although little explored scientifically. Despite being a rich source of phenolic compounds responsible in part for the proven anti-inflammatory activity, its characterization has not been carried out to date. The present work deals with the exhaustive identification and quantification of its phenolic fraction, along with its antioxidant activity. Decoction resulting from the bark as fine powder was filtered and lyophilized, and polyphenols were extracted from the resulting product by aqueous-organic solvents. Seventy-nine polyphenols were identified using LC-MSn. Hydroxycinnamates was the most abundant group of compounds (up to 66.8%), followed by hydroxycoumarins (15.5%), lignans (6.1%), flavonols (5.7%), phenolic simples (3.1), gallates (2.3%), flavanols (0.3%), and flavanones (0.2%). About 64% of the characterized phenols were in their glycosylated forms. The quantification of these phytochemicals by LC-QToF showed that this medicinal plant contained 2014.71 mg of phenolic compounds in 100 g dry matter, which evidences a great antioxidant potency determined by ABTS and DPPH assays. Therefore, Brunfelsia grandiflora represents an important source of polyphenols which supports its therapeutic properties scientifically proven.

Original languageEnglish
Article number6510
Issue number19
StatePublished - Oct 2022

Bibliographical note

Funding Information:
This research was funded by the Project “PCONFIGI A20041391” from the Major National University of San Marcos and the project 202270E021 funded by CSIC.

Publisher Copyright:
© 2022 by the authors.


  • Brunfelsia grandiflora
  • LC-MS-QToF
  • antioxidant activity
  • hydroxycoumarins
  • medicinal plant
  • polyphenols


Dive into the research topics of 'Identification, Quantification, and Characterization of the Phenolic Fraction of Brunfelsia grandiflora: In Vitro Antioxidant Capacity'. Together they form a unique fingerprint.

Cite this