Efecto citotóxico y genotóxico in vitro del extracto crudo y etanólico del rizoma de curcuma longa l.

Translated title of the contribution: In vitro cytotoxic and genotoxic effect of the crude and ethanolic extract from the rhizome of curcuma longa l.

Martha Francisca Cosquillo-Rafael, Maritza D. Placencia-Medina, Tomás Y. Miranda-Tomasevich, Miriam Moreno-Hinojosa, Mónica G. Retuerto-Figueroa

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To determine the in vitro cytotoxic and genotoxic effect of the crude and ethanolic extract from the Curcuma longa L. rhizome. Materials and methods: The cytotoxic effect was evaluated using DU-145, HT-29, 3T3 BALB/c cell lines. The growth percentages in 48 hours; and the half maximal in-hibitory concentration (IC50 ) were determined. The genotoxic effect on human genomic DNA was determined using the Tomasevich method. Results: Crude extract produced an IC50 of 12.98 ± 0.21 μg/mL for the HT-29 tumor cell line, which is lower than the value obtained for DU-145, with an IC50 of 36.77 ± 9.12 μg/mL. The ethanolic extract presented an IC50 of 13.24 ± 0.77 and 20.54 ± 2.58 μg/mL for both cell lines, respectively; the curcumin standard compound presented an IC50 of 3.96 ± 0.60 and 13.94 ± 2.79 μg/mL, respectively. Crude extract concentrations of 50 and 100 mg/mL fragmented between 40% to 95% of human genomic DNA; while at 200 mg/mL, fragmentation was greater than 95%. The ethan-olic extract at all concentrations did not fragment the DNA. Curcumin at 200 mg/mL fragmented less than 5% of human genomic DNA. Conclusions: The crude and ethanolic extracts of Curcuma longa L. demonstrate different in vitro cytotoxic effects for the human tumor cell lines DU-145 and HT-29; simi-lar to the standard curcumin compound. The crude extract of Curcuma longa L. shows a potent genotoxic in vitro activity against human genomic DNA; this type of effect is not produced by the ethanolic extract.

Translated title of the contributionIn vitro cytotoxic and genotoxic effect of the crude and ethanolic extract from the rhizome of curcuma longa l.
Original languageSpanish
Pages (from-to)454-461
Number of pages8
JournalRevista Peruana de Medicina de Experimental y Salud Publica
Volume37
Issue number3
DOIs
StatePublished - 1 Jul 2020
Externally publishedYes

Bibliographical note

Funding Information:
Agradecimientos: Al Dr. Abraham J. Vaisberg Wolach, profesor principal del Departamento de Microbiolog?a e investigador de la Unidad de Biolog?a Celular y Virolog?a de la Universidad Peruana Cayetano Heredia, por su valiosa colaboraci?n.

Publisher Copyright:
© 2020, Instituto Nacional de Salud. All rights reserved.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

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