Expresión in vitro de las interleucinas 2 y 10 de linfocitos de alpacas (Vicugna pacos) en presencia de antígenos clostridiales

Translated title of the contribution: In vitro expression of interleukin-2 and -10 from alpaca (Vicugna pacos) leukocytes in presence of clostridial antigens

W. Raquel Watanabe, S. Alberto Manchego, Hermelinda Rivera Geronimo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The aim of the study was to determine the relative in vitro ARNm expression levels of IL-2 and IL-10 in peripheral blood leukocytes by real time RT-PCR and relative quantification using the 2-AACt method in presence of clostridial antigens and GAPDH as an endogenous control. Whole blood was collected from 10 adult alpacas. Samples were centrifuged to obtain leukocytes using the Ficoll reagent, purified with ammonium chloride and cultured in 24-well plates at a concentration of 500 000 live cells/ml minimal essential medium (MEM) with clostridial extract suspensions at concentrations of 400, 16, 0.8 and 0.2 μg/ml. Subsequently, the RT-qPCR test was performed. IL-2 kinetic expression patterns were inversely proportional to the clostridial antigen dose in the three incubation times (p>0.05), unlike IL-10 which its kinetic expression patterns were variable, reaching its maximum at 12 h (p<0.05) while showing a similar trend like IL-2 in the 16 μg/ml clostridial antigen dose. IL-10 expression exceeded 40-fold the control expression respect to IL-2. A strong polarization towards the Th2 subtype was shown.

Translated title of the contributionIn vitro expression of interleukin-2 and -10 from alpaca (Vicugna pacos) leukocytes in presence of clostridial antigens
Original languageSpanish
Pages (from-to)419-429
Number of pages11
JournalRevista de Investigaciones Veterinarias del Peru
Volume25
Issue number3
StatePublished - 2014
Externally publishedYes

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