TY - JOUR
T1 - Interleukin 10 (IL-10) and transforming growth factor beta (TGF-β) expression in intestinal mucosa of alpaca crias (Vicugna Pacos)
AU - Cesar, Burga C.
AU - Alberto, Manchego S.
AU - Gina, Castro S.
AU - Giovanni, Pérez G.
AU - Mercy, Ramírez V.
AU - Nieves, Sandoval C.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - The aim of the present study was to evaluate the relative expression levels of cytokines IL-10 and TGF-β in the intestinal mucosa of clinically healthy alpacas from 2 to 47 days old. The animals were classified in three age groups (six animals per group): 2-8 days old (group 1), 10-21 days old (group 2) and 26-47-days old (group 3), regardless sex or breed. The midportion of jejunum of each animal was collected; total RNA was extracted and then analyzed by real-time RT-PCR technique using IL-10 and TGF-β specific primers. The relative mRNA expression analysis was done using the comparative 2-δδCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene and jejunum of three 11-month-old fetuses were used as calibrators. The results showed that IL-10 mRNA expression levels were 7.21±1.02 (group 1), 13.53±1.26 (group 2), and 18.77±1.48 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). Also, TGF-β mRNA expression levels were 2.18±0.23 (group 1), 3.03±0.18 (group 2), and 4.06±0.15 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). The expression of IL-10 and TGF-β mRNA was age dependent, increasing significantly in older animals.
AB - The aim of the present study was to evaluate the relative expression levels of cytokines IL-10 and TGF-β in the intestinal mucosa of clinically healthy alpacas from 2 to 47 days old. The animals were classified in three age groups (six animals per group): 2-8 days old (group 1), 10-21 days old (group 2) and 26-47-days old (group 3), regardless sex or breed. The midportion of jejunum of each animal was collected; total RNA was extracted and then analyzed by real-time RT-PCR technique using IL-10 and TGF-β specific primers. The relative mRNA expression analysis was done using the comparative 2-δδCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene and jejunum of three 11-month-old fetuses were used as calibrators. The results showed that IL-10 mRNA expression levels were 7.21±1.02 (group 1), 13.53±1.26 (group 2), and 18.77±1.48 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). Also, TGF-β mRNA expression levels were 2.18±0.23 (group 1), 3.03±0.18 (group 2), and 4.06±0.15 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). The expression of IL-10 and TGF-β mRNA was age dependent, increasing significantly in older animals.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85028809897&origin=inward
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85028809897&origin=inward
U2 - 10.15381/rivep.v28i2.13082
DO - 10.15381/rivep.v28i2.13082
M3 - Article
SN - 1682-3419
SP - 439
EP - 447
JO - Revista de Investigaciones Veterinarias del Peru
JF - Revista de Investigaciones Veterinarias del Peru
ER -