Isolation and characterization of novel microsatellite markers for Arctium minus (Compositae)

Sara López-Vinyallonga; Mónica Arakaki; Núria Garcia-Jacas; Alfonso Susanna; Matthew A. Gitzendanner; Douglas E. Soltis; Pamela S. Soltis

doi:10.3732/ajb.0900376

Isolation and characterization of novel microsatellite markers for Arctium minus (Compositae)

Sara López-Vinyallonga, Mónica Arakaki, Núria Garcia-Jacas, Alfonso Susanna, Matthew A. Gitzendanner, Douglas E. Soltis, Pamela S. Soltis

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Premise of the study: Microsatellite primers were developed for the invasive plant Arctium minus to investigate the effects of facultative self-pollination and the biannual habit on population genetic structure, as well as the colonization of the Americas by this Eurasian species. Methods and Results: Sixteen di- and trinucleotide microsatellite loci were identified in six populations. The number of alleles per locus ranged from one to 10, observed heterozygosities ranged from 0.000 to 0.897, and the mean value of FIS was 0.316. Conclusions: These results indicate the utility of these loci in future studies of population genetics in A. minus.

Original language	English
Pages (from-to)	e4-e6
Journal	American Journal of Botany
Volume	97
Issue number	2
DOIs	https://doi.org/10.3732/ajb.0900376
State	Published - Feb 2010
Externally published	Yes

Keywords

Arctium minus
Enriched library
Lesser burdock
Microsatellites
Population genetics
SSR

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10.3732/ajb.0900376

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title = "Isolation and characterization of novel microsatellite markers for Arctium minus (Compositae)",

abstract = "Premise of the study: Microsatellite primers were developed for the invasive plant Arctium minus to investigate the effects of facultative self-pollination and the biannual habit on population genetic structure, as well as the colonization of the Americas by this Eurasian species. Methods and Results: Sixteen di- and trinucleotide microsatellite loci were identified in six populations. The number of alleles per locus ranged from one to 10, observed heterozygosities ranged from 0.000 to 0.897, and the mean value of FIS was 0.316. Conclusions: These results indicate the utility of these loci in future studies of population genetics in A. minus.",

keywords = "Arctium minus, Enriched library, Lesser burdock, Microsatellites, Population genetics, SSR",

author = "Sara L{\'o}pez-Vinyallonga and M{\'o}nica Arakaki and N{\'u}ria Garcia-Jacas and Alfonso Susanna and Gitzendanner, {Matthew A.} and Soltis, {Douglas E.} and Soltis, {Pamela S.}",

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T1 - Isolation and characterization of novel microsatellite markers for Arctium minus (Compositae)

AU - López-Vinyallonga, Sara

AU - Arakaki, Mónica

AU - Garcia-Jacas, Núria

AU - Susanna, Alfonso

AU - Gitzendanner, Matthew A.

AU - Soltis, Douglas E.

AU - Soltis, Pamela S.

PY - 2010/2

Y1 - 2010/2

N2 - Premise of the study: Microsatellite primers were developed for the invasive plant Arctium minus to investigate the effects of facultative self-pollination and the biannual habit on population genetic structure, as well as the colonization of the Americas by this Eurasian species. Methods and Results: Sixteen di- and trinucleotide microsatellite loci were identified in six populations. The number of alleles per locus ranged from one to 10, observed heterozygosities ranged from 0.000 to 0.897, and the mean value of FIS was 0.316. Conclusions: These results indicate the utility of these loci in future studies of population genetics in A. minus.

AB - Premise of the study: Microsatellite primers were developed for the invasive plant Arctium minus to investigate the effects of facultative self-pollination and the biannual habit on population genetic structure, as well as the colonization of the Americas by this Eurasian species. Methods and Results: Sixteen di- and trinucleotide microsatellite loci were identified in six populations. The number of alleles per locus ranged from one to 10, observed heterozygosities ranged from 0.000 to 0.897, and the mean value of FIS was 0.316. Conclusions: These results indicate the utility of these loci in future studies of population genetics in A. minus.

KW - Arctium minus

KW - Enriched library

KW - Lesser burdock

KW - Microsatellites

KW - Population genetics

KW - SSR

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Isolation and characterization of novel microsatellite markers for Arctium minus (Compositae)

Abstract

Keywords

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