TY - JOUR
T1 - Estudio lectinhistoquímico del útero de alpacas (Vicugna pacos) bajo tratamiento superovulatorio
AU - Verónica, López C.
AU - Vasquez Cachay, Maria Elith
AU - Ramos Coaguila, Olger Pedro
AU - Santiani Acosta, Alexei Vicent
AU - Barbeito, Claudio
AU - Canuzzi, Carolina
AU - Lira Mejia, Boris Antonio
AU - Rodriguez Gutierrez, Jose Luis
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - The aim of this study was to determine the glycosylation pattern in the uterine endometrium of alpacas with and without superovulation treatment. Uterine tissues were obtained from five non-treated alpacas with ovarian follicles less than 4 mm andfive superovulated alpacas where FSH, GnRH and hCG was used. Samples from the uterus and uterine horns were analyzed by histochemistry (PAS and alcian blue) and lectinhistochemistry techniques. Fourteen lectins were used and PNA, RCA-1, ConA, DBA, WGA, Swga, GS, LCA, SJA and PHA-L showed different glycosilation patterns in the endometrium between both groups, indicating that the hormonal treatment could affect the expression of glycoconjugates that have affinity for these lectins. Lectins PNA, SJA, SBA, ConA, LCA and PSA showed different glycosilation patterns between the two uterine horns, indicating that the glycoconjugates that have affinity for these lectins can be implicated in the uterine environment during the implantation, which in most cases in the alpaca take place on the left horn. In conclusion, the glycosylation pattern in the alpaca can change due to superovulation hormone therapy.
AB - The aim of this study was to determine the glycosylation pattern in the uterine endometrium of alpacas with and without superovulation treatment. Uterine tissues were obtained from five non-treated alpacas with ovarian follicles less than 4 mm andfive superovulated alpacas where FSH, GnRH and hCG was used. Samples from the uterus and uterine horns were analyzed by histochemistry (PAS and alcian blue) and lectinhistochemistry techniques. Fourteen lectins were used and PNA, RCA-1, ConA, DBA, WGA, Swga, GS, LCA, SJA and PHA-L showed different glycosilation patterns in the endometrium between both groups, indicating that the hormonal treatment could affect the expression of glycoconjugates that have affinity for these lectins. Lectins PNA, SJA, SBA, ConA, LCA and PSA showed different glycosilation patterns between the two uterine horns, indicating that the glycoconjugates that have affinity for these lectins can be implicated in the uterine environment during the implantation, which in most cases in the alpaca take place on the left horn. In conclusion, the glycosylation pattern in the alpaca can change due to superovulation hormone therapy.
KW - Alpaca
KW - Endometrium
KW - Glycosylation pattern
KW - Lectins
KW - Superovulation
UR - http://www.scopus.com/inward/record.url?scp=84896994766&partnerID=8YFLogxK
M3 - Artículo
AN - SCOPUS:84896994766
VL - 25
SP - 1
EP - 15
JO - Revista de Investigaciones Veterinarias del Peru
JF - Revista de Investigaciones Veterinarias del Peru
SN - 1682-3419
IS - 1
ER -