| Original language | English |
|---|---|
| Pages (from-to) | 60 |
| Number of pages | 1 |
| Journal | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| Volume | 85 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1991 |
| Externally published | Yes |
Bibliographical note
Funding Information:Leishmania 4-10 d later. The isolate was coded MMES/PE/88/Centinela. Previous reports on the vectors of leishmaniases in Peni were based on the predominance of Lu. vewucar-urn and Lu. peruensis within endemic areas, the isolation of Leishmania sp. in a hamster inoculated with a triturated pool of Lu. peruensis, Leishmania infections in sentinel hamsters placed in sandfly resting sites (HERRER1 977, 1982a, 1982b), and the isolation of Leishmania sp. in culture from Lu. peruensis( CRUZADO1, 987). None of these isolates was characterized biochemically. The polymerase chain reaction, using a specific sequenceo f kinetoplast deoxyribonucleic acid (LOPEZ et al., 1990), and isoenzyme electrophoresis in thin starch gel and cellulose acetate using 5 different enzymes, including mannose phosphate isomerase (E.C.5.3.1.8) which distinguishes L. (V.) peruviana from L. (V.) braziliensis (ARANA et al., l!Wl), was used to characterize our isolates. They were identified as L. (V.) peruviana, as were others from the same locality obtained from Didelphis albiventris and patients suffering from uta. The same species of Leishmania was isolated using sentinel hamsters placed in resting sites, where Lu. peruensis, being present in high density, is the most probable vector. We thank JaimeC hangf or his comments and reviewing the manuscript. This study received financial support from the International Development Research Centre, Canada, project No. 3-P-85-1043-02.