Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Hector H. Garcia; Yesenia Castillo; Isidro Gonzales; Javier A. Bustos; Herbert Saavedra; Louis Jacob; Oscar H. Del Brutto; Patricia P. Wilkins; Armando E. Gonzalez; Robert H. Gilman; Victor C.W. Tsang; Silvia Rodriguez; Manuel Martinez; Manuel Alvarado; Miguel Porras; Victor Vargas; Alfredo Ccjuno; Manuela Verastegui; Mirko Zimic; Holger Mayta; Cristina Guerra; Yagahira Castro; Maria T. Lopez; Cesar M. Gavidia; Luis Gomez; Luz M. Moyano; Ricardo Gamboa; Claudio Muro; Percy Vilchez; Theodore E. Nash; Siddhartha Mahanty; John Noh; Sukwan Handali; Jon Friedland

doi:10.1111/tmi.13010

Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Hector H. Garcia, Yesenia Castillo, Isidro Gonzales, Javier A. Bustos, Herbert Saavedra, Louis Jacob, Oscar H. Del Brutto, Patricia P. Wilkins, Armando E. Gonzalez, Robert H. Gilman, Victor C.W. Tsang, Silvia Rodriguez, Manuel Martinez, Manuel Alvarado, Miguel Porras, Victor Vargas, Alfredo Ccjuno, Manuela Verastegui, Mirko Zimic, Holger MaytaCristina Guerra, Yagahira Castro, Maria T. Lopez, Cesar M. Gavidia, Luis Gomez, Luz M. Moyano, Ricardo Gamboa, Claudio Muro, Percy Vilchez, Theodore E. Nash, Siddhartha Mahanty, John Noh, Sukwan Handali, Jon Friedland

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

© 2017 John Wiley & Sons Ltd Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

Original language	American English
Pages (from-to)	101-105
Number of pages	5
Journal	Tropical Medicine and International Health
DOIs	https://doi.org/10.1111/tmi.13010
State	Published - 1 Jan 2018

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Garcia, H. H., Castillo, Y., Gonzales, I., Bustos, J. A., Saavedra, H., Jacob, L., Del Brutto, O. H., Wilkins, P. P., Gonzalez, A. E., Gilman, R. H., Tsang, V. C. W., Rodriguez, S., Martinez, M., Alvarado, M., Porras, M., Vargas, V., Ccjuno, A., Verastegui, M., Zimic, M., ... Friedland, J. (2018). Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis. Tropical Medicine and International Health, 101-105. https://doi.org/10.1111/tmi.13010

Garcia, Hector H. ; Castillo, Yesenia ; Gonzales, Isidro ; Bustos, Javier A. ; Saavedra, Herbert ; Jacob, Louis ; Del Brutto, Oscar H. ; Wilkins, Patricia P. ; Gonzalez, Armando E. ; Gilman, Robert H. ; Tsang, Victor C.W. ; Rodriguez, Silvia ; Martinez, Manuel ; Alvarado, Manuel ; Porras, Miguel ; Vargas, Victor ; Ccjuno, Alfredo ; Verastegui, Manuela ; Zimic, Mirko ; Mayta, Holger ; Guerra, Cristina ; Castro, Yagahira ; Lopez, Maria T. ; Gavidia, Cesar M. ; Gomez, Luis ; Moyano, Luz M. ; Gamboa, Ricardo ; Muro, Claudio ; Vilchez, Percy ; Nash, Theodore E. ; Mahanty, Siddhartha ; Noh, John ; Handali, Sukwan ; Friedland, Jon. / Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis. In: Tropical Medicine and International Health. 2018 ; pp. 101-105.

@article{59b2707d12f04de0b99cbb44bd3c5e78,

title = "Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis",

abstract = "{\textcopyright} 2017 John Wiley & Sons Ltd Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa{\textregistered} and Ridascreen{\textregistered}, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa{\textregistered} and Ridascreen{\textregistered}. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa{\textregistered} and Ridascreen{\textregistered} was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.",

author = "Garcia, {Hector H.} and Yesenia Castillo and Isidro Gonzales and Bustos, {Javier A.} and Herbert Saavedra and Louis Jacob and {Del Brutto}, {Oscar H.} and Wilkins, {Patricia P.} and Gonzalez, {Armando E.} and Gilman, {Robert H.} and Tsang, {Victor C.W.} and Silvia Rodriguez and Manuel Martinez and Manuel Alvarado and Miguel Porras and Victor Vargas and Alfredo Ccjuno and Manuela Verastegui and Mirko Zimic and Holger Mayta and Cristina Guerra and Yagahira Castro and Lopez, {Maria T.} and Gavidia, {Cesar M.} and Luis Gomez and Moyano, {Luz M.} and Ricardo Gamboa and Claudio Muro and Percy Vilchez and Nash, {Theodore E.} and Siddhartha Mahanty and John Noh and Sukwan Handali and Jon Friedland",

year = "2018",

month = jan,

day = "1",

doi = "10.1111/tmi.13010",

language = "American English",

pages = "101--105",

journal = "Tropical Medicine and International Health",

issn = "1360-2276",

publisher = "Wiley-Blackwell Publishing Ltd",

}

Garcia, HH, Castillo, Y, Gonzales, I, Bustos, JA, Saavedra, H, Jacob, L, Del Brutto, OH, Wilkins, PP, Gonzalez, AE, Gilman, RH, Tsang, VCW, Rodriguez, S, Martinez, M, Alvarado, M, Porras, M, Vargas, V, Ccjuno, A, Verastegui, M, Zimic, M, Mayta, H, Guerra, C, Castro, Y, Lopez, MT, Gavidia, CM, Gomez, L, Moyano, LM, Gamboa, R, Muro, C, Vilchez, P, Nash, TE, Mahanty, S, Noh, J, Handali, S & Friedland, J 2018, 'Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis', Tropical Medicine and International Health, pp. 101-105. https://doi.org/10.1111/tmi.13010

Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis. / Garcia, Hector H.; Castillo, Yesenia; Gonzales, Isidro; Bustos, Javier A.; Saavedra, Herbert; Jacob, Louis; Del Brutto, Oscar H.; Wilkins, Patricia P.; Gonzalez, Armando E.; Gilman, Robert H.; Tsang, Victor C.W.; Rodriguez, Silvia; Martinez, Manuel; Alvarado, Manuel; Porras, Miguel; Vargas, Victor; Ccjuno, Alfredo; Verastegui, Manuela; Zimic, Mirko; Mayta, Holger; Guerra, Cristina; Castro, Yagahira; Lopez, Maria T.; Gavidia, Cesar M.; Gomez, Luis; Moyano, Luz M.; Gamboa, Ricardo; Muro, Claudio; Vilchez, Percy; Nash, Theodore E.; Mahanty, Siddhartha; Noh, John; Handali, Sukwan; Friedland, Jon.

In: Tropical Medicine and International Health, 01.01.2018, p. 101-105.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

AU - Garcia, Hector H.

AU - Castillo, Yesenia

AU - Gonzales, Isidro

AU - Bustos, Javier A.

AU - Saavedra, Herbert

AU - Jacob, Louis

AU - Del Brutto, Oscar H.

AU - Wilkins, Patricia P.

AU - Gonzalez, Armando E.

AU - Gilman, Robert H.

AU - Tsang, Victor C.W.

AU - Rodriguez, Silvia

AU - Martinez, Manuel

AU - Alvarado, Manuel

AU - Porras, Miguel

AU - Vargas, Victor

AU - Ccjuno, Alfredo

AU - Verastegui, Manuela

AU - Zimic, Mirko

AU - Mayta, Holger

AU - Guerra, Cristina

AU - Castro, Yagahira

AU - Lopez, Maria T.

AU - Gavidia, Cesar M.

AU - Gomez, Luis

AU - Moyano, Luz M.

AU - Gamboa, Ricardo

AU - Muro, Claudio

AU - Vilchez, Percy

AU - Nash, Theodore E.

AU - Mahanty, Siddhartha

AU - Noh, John

AU - Handali, Sukwan

AU - Friedland, Jon

PY - 2018/1/1

Y1 - 2018/1/1

N2 - © 2017 John Wiley & Sons Ltd Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

AB - © 2017 John Wiley & Sons Ltd Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

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DO - 10.1111/tmi.13010

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JO - Tropical Medicine and International Health

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