Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Hector H. Garcia, Yesenia Castillo, Isidro Gonzales, Javier A. Bustos, Herbert Saavedra, Louis Jacob, Oscar H. Del Brutto, Patricia P. Wilkins, Armando E. Gonzalez, Robert H. Gilman, Victor C.W. Tsang, Silvia Rodriguez, Manuel Martinez, Manuel Alvarado, Miguel Porras, Victor Vargas, Alfredo Ccjuno, Manuela Verastegui, Mirko Zimic, Holger MaytaCristina Guerra, Yagahira Castro, Maria T. Lopez, Cesar M. Gavidia, Luis Gomez, Luz M. Moyano, Ricardo Gamboa, Claudio Muro, Percy Vilchez, Theodore E. Nash, Siddhartha Mahanty, John Noh, Sukwan Handali, Jon Friedland

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

© 2017 John Wiley & Sons Ltd Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.
Original languageAmerican English
Pages (from-to)101-105
Number of pages5
JournalTropical Medicine and International Health
DOIs
StatePublished - 1 Jan 2018

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