Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

the Cysticercosis Working Group in Peru

doi:10.1111/tmi.13010

Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

the Cysticercosis Working Group in Peru

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa^® and Ridascreen^®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa^® and Ridascreen^®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa^® and Ridascreen^® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

Original language	English
Pages (from-to)	101-105
Number of pages	5
Journal	Tropical Medicine and International Health
Volume	23
Issue number	1
DOIs	https://doi.org/10.1111/tmi.13010
State	Published - Jan 2018

Bibliographical note

Funding Information:
We are very grateful to Y. Santos for her hard work on sample processing. Other members of the CWGP include Victor C.W.Tsang, PhD (Coordination Board); Silvia Rodriguez, MSc; Manuel Martinez, MD; Manuel Alvar-ado, MD; Miguel Porras, MD; Victor Vargas, MD; Alfredo Ccjuno, MD (Instituto Nacional de Ciencias Neurológicas, Lima, Perú); Manuela Verastegui, PhD; Mirko Zimic, PhD; Holger Mayta, PhD; Cristina Guerra, PhD; Yesenia Castillo, MSc; Yagahira Castro, MSc (Universidad Peruana Cayetano Heredia, Lima, Perú); Maria T. Lopez, DVM, PhD; Cesar M. Gavidia, DVM, PhD; Luis Gomez, DVM (School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú); Luz M. Moyano, MD; Ricardo Gamboa, MSc; Claudio Muro, Percy Vilchez, MSc (Cysticercosis Elimination Program, Tumbes, Perú); Theodore E. Nash, MD; Siddhartha Mahanty, MD, PhD (NIAID, NIH, Bethesda, MD); John Noh, BS; Sukwan Handali, MD (CDC, Atlanta, GA); Jon Friedland (Imperial College, London, UK). This study was partially supported by grant D43TW001140 from the Fogarty International Center of the US National Institutes of Health (YC, JAB).

Publisher Copyright:
© 2017 John Wiley & Sons Ltd

Keywords

ELISA
Peru
Taenia solium
antibody
cysticercosis
neurocysticercosis

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10.1111/tmi.13010

Cite this

@article{e0c6411eb66042b7a4a54d03896fdbde,

title = "Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis",

abstract = "Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa{\textregistered} and Ridascreen{\textregistered}, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa{\textregistered} and Ridascreen{\textregistered}. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa{\textregistered} and Ridascreen{\textregistered} was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.",

keywords = "ELISA, Peru, Taenia solium, antibody, cysticercosis, neurocysticercosis",

author = "{the Cysticercosis Working Group in Peru} and Garcia, {Hector H.} and Yesenia Castillo and Isidro Gonzales and Bustos, {Javier A.} and Herbert Saavedra and Louis Jacob and {Del Brutto}, {Oscar H.} and Wilkins, {Patricia P.} and Gonzalez, {Armando E.} and Gilman, {Robert H.} and Tsang, {Victor C.W.} and Silvia Rodriguez and Manuel Martinez and Manuel Alvarado and Miguel Porras and Victor Vargas and Alfredo Ccjuno and Manuela Verastegui and Mirko Zimic and Holger Mayta and Cristina Guerra and Yagahira Castro and Lopez, {Maria T.} and Gavidia, {Cesar M.} and Luis Gomez and Moyano, {Luz M.} and Ricardo Gamboa and Claudio Muro and Percy Vilchez and Nash, {Theodore E.} and Siddhartha Mahanty and John Noh and Sukwan Handali and Jon Friedland",

note = "Publisher Copyright: {\textcopyright} 2017 John Wiley & Sons Ltd",

year = "2018",

month = jan,

doi = "10.1111/tmi.13010",

language = "Ingl{\'e}s",

volume = "23",

pages = "101--105",

journal = "Tropical Medicine and International Health",

issn = "1360-2276",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

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TY - JOUR

T1 - Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

AU - the Cysticercosis Working Group in Peru

AU - Garcia, Hector H.

AU - Castillo, Yesenia

AU - Gonzales, Isidro

AU - Bustos, Javier A.

AU - Saavedra, Herbert

AU - Jacob, Louis

AU - Del Brutto, Oscar H.

AU - Wilkins, Patricia P.

AU - Gonzalez, Armando E.

AU - Gilman, Robert H.

AU - Tsang, Victor C.W.

AU - Rodriguez, Silvia

AU - Martinez, Manuel

AU - Alvarado, Manuel

AU - Porras, Miguel

AU - Vargas, Victor

AU - Ccjuno, Alfredo

AU - Verastegui, Manuela

AU - Zimic, Mirko

AU - Mayta, Holger

AU - Guerra, Cristina

AU - Castro, Yagahira

AU - Lopez, Maria T.

AU - Gavidia, Cesar M.

AU - Gomez, Luis

AU - Moyano, Luz M.

AU - Gamboa, Ricardo

AU - Muro, Claudio

AU - Vilchez, Percy

AU - Nash, Theodore E.

AU - Mahanty, Siddhartha

AU - Noh, John

AU - Handali, Sukwan

AU - Friedland, Jon

PY - 2018/1

Y1 - 2018/1

N2 - Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

AB - Objective: To evaluate the diagnostic performance of two commercially available ELISA kits, Novalisa® and Ridascreen®, for the detection of antibodies to Taenia solium, compared to serological diagnosis of neurocysticercosis (NCC) by LLGP-EITB (electro-immunotransfer blot assay using lentil-lectin purified glycoprotein antigens). Methods: Archive serum samples from patients with viable NCC (n = 45) or resolved, calcified NCC (n = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, n = 45 and cystic hydatid disease, n = 45), were evaluated for cysticercosis antibody detection using two ELISA kits, Novalisa® and Ridascreen®. All NCC samples had previously tested positive, and all samples from heterologous infections were negative on LLGP-EITB for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits. Results: Compared to LLGP-EITB, the sensitivity of both ELISA assays to detect specific antibodies in patients with viable NCC was low (44.4% and 22.2%), and for calcified NCC, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross-reactive in both ELISA assays (38/45, 84.4%; and 25/45, 55.6%). Sera from patients with hymenolepiasis cross-reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%). Conclusions: The performance of Novalisa® and Ridascreen® was poor. Antibody ELISA detection cannot be recommended for the diagnosis of neurocysticercosis.

KW - ELISA

KW - Peru

KW - Taenia solium

KW - antibody

KW - cysticercosis

KW - neurocysticercosis

UR - http://www.scopus.com/inward/record.url?scp=85040132698&partnerID=8YFLogxK

U2 - 10.1111/tmi.13010

DO - 10.1111/tmi.13010

M3 - Artículo

C2 - 29160912

AN - SCOPUS:85040132698

SN - 1360-2276

VL - 23

SP - 101

EP - 105

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 1

ER -

Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis

Abstract

Bibliographical note

Keywords

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