TY - JOUR
T1 - Método rápido para la cuantificación de leucocitos sanguíneos y su utilidad en la evaluación del estado de salud en trucha arcoíris Oncorhynchus mykiss
AU - Alzamora-Gonzales, Libertad
AU - de Amat-Herbozo, Carolina
AU - Colona-Vallejos, Erasmo
AU - Cervantes-Aguilar, Elizabeth
AU - Velarde-Álvarez, Richard Dyer
AU - Aquino-Ortega, Ronald
AU - Aguilar-Luis, Miguel Ángel
N1 - Publisher Copyright:
© 2015, Escuela de Ciencias del Mar. All rights reserved.
PY - 2015/11
Y1 - 2015/11
N2 - The analysis of the ratio and number of blood cells is a very reliable way to assess the health status in humans and other species. The aim was to standardize a methodology for rapid quantification of blood leukocytes in rainbow trout Oncorhynchus mykiss. Samples of blood leukocytes obtained from a group of healthy juvenile trout maintained in the laboratory were processed. The samples were analyzed using a Scepter™ minicytometer, the results were compared with those obtained by flow cytometry. Histograms generated by both methods showed a peak corresponding to the population of lymphocytes/thrombocytes and one of monocyte/neutrophil. Non-significant differences were found between the results of both methods (P > 0.05). The method employing minicytometer was used to evaluate samples of trout from a fish farm, the profile obtained showed a significant decrease in the neutrophils consistent with clinical signs of disease observed in the tested specimens. These results indicate that the methodology is valid and useful in determining the concentrations and cell proportions in blood samples of rainbow trout and allow rapid analysis of its health status.
AB - The analysis of the ratio and number of blood cells is a very reliable way to assess the health status in humans and other species. The aim was to standardize a methodology for rapid quantification of blood leukocytes in rainbow trout Oncorhynchus mykiss. Samples of blood leukocytes obtained from a group of healthy juvenile trout maintained in the laboratory were processed. The samples were analyzed using a Scepter™ minicytometer, the results were compared with those obtained by flow cytometry. Histograms generated by both methods showed a peak corresponding to the population of lymphocytes/thrombocytes and one of monocyte/neutrophil. Non-significant differences were found between the results of both methods (P > 0.05). The method employing minicytometer was used to evaluate samples of trout from a fish farm, the profile obtained showed a significant decrease in the neutrophils consistent with clinical signs of disease observed in the tested specimens. These results indicate that the methodology is valid and useful in determining the concentrations and cell proportions in blood samples of rainbow trout and allow rapid analysis of its health status.
KW - Aquaculture
KW - Blood counts in fish
KW - Blood leukocytes
KW - Oncorhynchus mykiss
KW - Rainbow trout
UR - http://www.scopus.com/inward/record.url?scp=84949937919&partnerID=8YFLogxK
U2 - 10.3856/vol43-issue5-fulltext-22
DO - 10.3856/vol43-issue5-fulltext-22
M3 - Artículo
AN - SCOPUS:84949937919
SN - 0718-560X
VL - 43
SP - 1019
EP - 1023
JO - Latin American Journal of Aquatic Research
JF - Latin American Journal of Aquatic Research
IS - 5
ER -