Magnetic particles as matrix for enzyme immobilization have been used and due to the enzymatic derivative can be easily removed from the reaction mixture by a magnetic field. This work presents a study about the synthesis and characterization of iron phases into magnetic montmorillonite clay (mMMT) and magnetic diatomaceous earth (mDE) by 57Fe Mössbauer spectroscopy (MS), magnetic measurements and X-ray diffraction (XRD). Also these magnetic materials were assessed as matrices for the immobilization of invertase via covalent binding. Mössbauer spectra of the magnetic composites performed at 4.2 K showed a mixture of magnetite and maghemite about equal proportion in the mMMT, and a pure magnetite phase in the sample mDE. These results were verified using XRD. The residual specific activity of the immobilized invertase on mMMT and mDE were 83 % and 92.5 %, respectively. Thus, both magnetic composites showed to be promising matrices for covalent immobilization of invertase. © 2013 Springer Science+Business Media Dordrecht.
Cabrera, M., Maciel, J. C., Quispe-Marcatoma, J., Pandey, B., Neri, D. F. M., Soria, F., Baggio-Saitovitch, E., & de Carvalho, L. B. (2014). Magnetic composites from minerals: Study of the iron phases in clay and diatomite using Mössbauer spectroscopy, magnetic measurements and XRD. Hyperfine Interactions, 197-204. https://doi.org/10.1007/s10751-013-0858-x