Premise: Microsatellite primers were developed for Puya raimondii (Bromeliaceae), an endangered species distributed in the Andean Mountains of Bolivia and Peru. Methods and Results: Genome skimming of P. raimondii, P. macrura, and P. hutchisonii resulted in the selection of 46 pairs of cross-species microsatellite markers. Of these, 12 microsatellite primer pairs produced clear and polymorphic bands in P. raimondii. These primer sets were then used for the detection of potential polymorphisms in 84 P. raimondii individuals collected from four populations in Peru. The number of alleles per locus ranged from one to six, and the observed and expected levels of heterozygosity ranged from 0.000 to 0.8929 and from 0.000 to 0.7662, respectively. Conclusions: The microsatellite markers developed in this study will be useful for future population genetic analyses and breeding system studies in P. raimondii.
Bibliographical noteFunding Information:
The authors thank Dr. M?nica Arakaki for her help during the development of the work and Professor Asuncion Cano for field assistance and species identification. This study was financially supported by the International Partnership Program of the Chinese Academy of Sciences (grant no. GJHZ1620).
© 2019 Tumi et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America
- Puya raimondii
- codominant markers
- genetic variability
- genome skimming
- next-generation sequencing