Microsatellite markers for the endangered Puya raimondii in Peru

Liscely Tumi, Yu Qu Zhang, Zheng Feng Wang, Mery L. Suni, Kevin S. Burgess, Xue jun Ge

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Premise: Microsatellite primers were developed for Puya raimondii (Bromeliaceae), an endangered species distributed in the Andean Mountains of Bolivia and Peru. Methods and Results: Genome skimming of P. raimondii, P. macrura, and P. hutchisonii resulted in the selection of 46 pairs of cross-species microsatellite markers. Of these, 12 microsatellite primer pairs produced clear and polymorphic bands in P. raimondii. These primer sets were then used for the detection of potential polymorphisms in 84 P. raimondii individuals collected from four populations in Peru. The number of alleles per locus ranged from one to six, and the observed and expected levels of heterozygosity ranged from 0.000 to 0.8929 and from 0.000 to 0.7662, respectively. Conclusions: The microsatellite markers developed in this study will be useful for future population genetic analyses and breeding system studies in P. raimondii.

Original languageEnglish
Article numbere11308
JournalApplications in Plant Sciences
Volume7
Issue number12
DOIs
StatePublished - 1 Dec 2019

Bibliographical note

Funding Information:
The authors thank Dr. M?nica Arakaki for her help during the development of the work and Professor Asuncion Cano for field assistance and species identification. This study was financially supported by the International Partnership Program of the Chinese Academy of Sciences (grant no. GJHZ1620).

Publisher Copyright:
© 2019 Tumi et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America

Keywords

  • Bromeliaceae
  • Puya raimondii
  • codominant markers
  • genetic variability
  • genome skimming
  • next-generation sequencing

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