Modulation of functional activities of the neurotoxin from black widow spider venom

E. V. Grishin; N. H. Himmelreich; K. A. Pluzhnikov; N. G. Pozdnyakova; L. G. Storchak; T. M. Volkova; P. G. Woll

doi:10.1016/0014-5793(93)80803-3

Modulation of functional activities of the neurotoxin from black widow spider venom

E. V. Grishin, N. H. Himmelreich, K. A. Pluzhnikov, N. G. Pozdnyakova, L. G. Storchak, T. M. Volkova, P. G. Woll

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [¹⁴C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [¹⁴C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

Original language	English
Pages (from-to)	205-207
Number of pages	3
Journal	FEBS Letters
Volume	336
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(93)80803-3
State	Published - 27 Dec 1993
Externally published	Yes

Keywords

Calcium influx
GABA release
PC12 cell
Rat brain synaptosome
α-Latrotoxin

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10.1016/0014-5793(93)80803-3

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title = "Modulation of functional activities of the neurotoxin from black widow spider venom",

abstract = "We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.",

keywords = "Calcium influx, GABA release, PC12 cell, Rat brain synaptosome, α-Latrotoxin",

author = "Grishin, {E. V.} and Himmelreich, {N. H.} and Pluzhnikov, {K. A.} and Pozdnyakova, {N. G.} and Storchak, {L. G.} and Volkova, {T. M.} and Woll, {P. G.}",

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T1 - Modulation of functional activities of the neurotoxin from black widow spider venom

AU - Grishin, E. V.

AU - Himmelreich, N. H.

AU - Pluzhnikov, K. A.

AU - Pozdnyakova, N. G.

AU - Storchak, L. G.

AU - Volkova, T. M.

AU - Woll, P. G.

PY - 1993/12/27

Y1 - 1993/12/27

N2 - We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

AB - We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

KW - Calcium influx

KW - GABA release

KW - PC12 cell

KW - Rat brain synaptosome

KW - α-Latrotoxin

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DO - 10.1016/0014-5793(93)80803-3

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VL - 336

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ER -

Modulation of functional activities of the neurotoxin from black widow spider venom

Abstract

Keywords

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