Modulation of functional activities of the neurotoxin from black widow spider venom

E. V. Grishin, N. H. Himmelreich, K. A. Pluzhnikov, N. G. Pozdnyakova, L. G. Storchak, T. M. Volkova, Patricia Gloria Woll Toso

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

Original languageEnglish
Pages (from-to)205-207
Number of pages3
JournalFEBS Letters
Volume336
Issue number2
DOIs
StatePublished - 27 Dec 1993
Externally publishedYes

Keywords

  • Calcium influx
  • GABA release
  • PC12 cell
  • Rat brain synaptosome
  • α-Latrotoxin

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