Molecular characterization of the rabies virus’ nucleoprotein in dogs from Arequipa, Peru

Carina Rosario Mantari Torpoco, Alfredo Miguel Berrocal Huallpa, Abraham Omar Espinoza-Culupú, Ricardo Luis López-Ingunza

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

All rights reserved. Objective. To characterize the nucleoprotein (N) and establish the origin of the rabies virus in dogs coming from Arequipa. Materials and Methods. Thirty samples of nervous tissue from the departments of Arequipa and Puno were analyzed. Total RNA was extracted from the samples and cDNA was synthesized to amplify the nucleoprotein gene, sequence it, and perform bioinformatics analysis. Results. A defined group was formed with respect to the external group (European bat lyssavirus). This group was classified into two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), exhibiting a nucleotide identity of 99.9% in subgroup A. This group was classified in two subgroups, one constituted by samples coming from Puno and Arequipa (subgroup A), and another one by samples from Puno (subgroup B), observing a nucleotide identity of 99.9% in subgroup A. Conclusions. The groupings of viral sequences show that the cases of canine rabies reported in Arequipa are the result of the expansion of canine rabies from the endemic region of Puno.
Original languageAmerican English
Pages (from-to)46-53
Number of pages8
JournalRevista Peruana de Medicina Experimental y Salud Publica
Volume36
Issue number1
DOIs
StatePublished - 1 Mar 2019

Keywords

  • Dogs
  • High-performance nucleotide sequencing
  • Peru
  • Rabies virus
  • RNA virus

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