Molecular epidemiology of coronavirus in faeces of brazilian calves and peruvian camelid herds

Camila Banks Rocha, Luz Alba Maria Garcete Fornells, Miguel Rojas, Maíra Liberal, Alberto Manchego, Danilo Pezo, Norma Santos

Research output: Contribution to journalArticle

Abstract

© 2018 Rocha et al. Introduction: The enteric disorders represent a serious hazard for bovine and camelid breeding. The aim of this study was to examine the frequency of detection and molecular characteristics of enteric coronavirus (CoV) infections in cattle, alpaca, and llama herds bred in family-based farms in Brazil and Peru. Methodology: Stool samples were collected from calves from Brazil and camelids from Peru for detection and characterization of CoV by reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis. Results: 46.5% (47/101) samples from calves and 26.8% (70/261) from alpaca tested positive for CoV. All strains belong to lineage A1 of the Betacoronavirus genus. Phylogenetic analysis showed high identity between CoV strains detected in calves and alpacas. Conclusions: This study characterised CoV strains from dairy cattle herds in the state of Rio de Janeiro, Brazil, and indicated that this virus is spread among the state herds. The results also indicate widespread circulation of CoV among the alpacas of Cuzco, Peru.
Original languageAmerican English
Pages (from-to)37-42
Number of pages6
JournalJournal of Infection in Developing Countries
DOIs
StatePublished - 1 Jan 2018

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