Molecular evidences of a hidden complex scenario in Leporinus cf. friderici

Rosane Silva-Santos, Jorge L. Ramirez, Pedro M. Galetti, Patrícia D. Freitas

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11 Scopus citations

Abstract

The megadiversity of the neotropical ichthyofauna has been associated to recent diversification processes, reflecting in subtle or lacking morphological differentiation between species, challenging the classical taxonomic identification. Leporinus friderici occurs in several river basins of South America, and its nominal taxonomic validity has been questioned. Its wide distribution within the Brazilian Shield suggests that this species could be genetically structured among the hydrographic basins, despite a sharp morphological similarity. In this study, we performed phylogenetic analyses, based on three nuclear (recombination activating gene 1, RAG1, recombination activating gene 2, RAG2, and myosin heavy chain 6 cardiac muscle alpha gene, Myh6) and two mitochondrial (COI and Cytochrome b, Cytb) markers, in specimens morphologically similar to L. friderici and related species from different hydrographic basins in South America. Our phylogenetic tree identified four well-supported clades, which point out to the existence of taxonomic inconsistencies within this fish group. A clade named L. cf. friderici sensu stricto included eight Molecular Operational Taxonomic Units recently diversified in the Brazilian Shield basins. These results were also confirmed by a single-gene species delimitation analysis. It is suggested that this clade includes a species complex, characterizing taxonomic uncertainties. Another clade recovered only L. friderici from the Suriname rivers, validating this nominal species in its type locality. A third no-named clade, characterized by deeper species divergence, recovered five different nominal species interleaved with other undescribed forms previously also recognized as L. cf. friderici, indicating taxonomic errors. The fourth clade only included L. taeniatus. Our results showed a complex scenario involving the morphotype L. cf. friderici and allowed us to address aspects related to evolutionary diversification of this fish group and historical processes involved with, highlighting the importance of revealing hidden biodiversity for the taxonomy and conservationist action plans of these fish.

Original languageEnglish
Article number47
JournalFrontiers in Genetics
Volume9
Issue numberFEB
DOIs
StatePublished - 15 Feb 2018

Bibliographical note

Funding Information:
We are grateful to C. Cramer, C. Doria, D. Carvalho, H. Ortega, J. A. Rodriguez, J. C. Riofrio, P. Venere, W. Troy, and U. Lopes for help to obtain part of the tissue or DNA samples and ICMBIO/MMA for sampling fish authorization (32215-1). We also thank H. Britski, P. Venere, J. Zuanon, N. Priorski, and H. Ortega for the specimen identification and the two reviewers for their comments and suggestions, which have improved the manuscript. This study was supported by Conselho Nacional de Desenvolvimento Cientif?co e Tecnol?gico (CNPq, 304440/2009-4 and 473474/2011-5), SISBIOTA-Brazil Program (CNPq, 563299/2010-0; FAPESP, 10/52315-7), Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES, n?mero to RS-S), and Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP, 2011/21836-4 to JR)

Publisher Copyright:
© 2018 Silva-Santos, Ramirez, Galetti and Freitas.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

  • Cryptic species
  • Fish phylogeny
  • MOTUs
  • Neotropical fish
  • Taxonomic uncertainties

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