Molecular fingerprinting of Campylobacter and Arcobacter isolated from chicken and

Ana González, María A. Ferrús, Rosa González, Javier Hernández

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The potential of a fingerprinting method based on the single-enzyme amplified fragment length polymorphism (s-AFLP) technique was evaluated for its efficacy in detecting foodborne Campylobacter and Arcobacter species. Campylobacter and Arcobacter isolates from chicken and water samples were subjected to s-AFLP and pulsed-field gel electrophoresis (PFGE) profiling. Molecular typing revealed a high degree of heterogeneity. AFLP was found to be appropriate for differentiating minimal genomic variations, which makes this technique a valuable tool for the identification of isolates. PFGE was effective in showing epidemiological relationships among closely related isolates. Either technique allowed the discrimination of A. butzleri from A. cryaerophilus and A. skirrowii. When used together, s-AFLP and PFGE can be applied to determine taxonomic and epidemiological relationships among campylobacteria.
Original languageAmerican English
Pages (from-to)85-90
Number of pages6
JournalInternational Microbiology
DOIs
StatePublished - 1 Jan 2007
Externally publishedYes

Fingerprint Dive into the research topics of 'Molecular fingerprinting of Campylobacter and Arcobacter isolated from chicken and'. Together they form a unique fingerprint.

  • Cite this