Identificación molecular de salmonella typhimurium en cuyes al primer parto mediante la técnica de PCR multiple

Translated title of the contribution: Molecular identification of salmonella typhimurium and enteritidis in Guinea pigs at first parturition by multiplex PCR

Chero O. Ana, Rosadio A. Raúl, Marcelo M. Geraldine, Díaz O. Gerardo, Ronald Jimenez Aliaga, Castro G. Yenny, Maturrano H. Lenin

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

Translated title of the contributionMolecular identification of salmonella typhimurium and enteritidis in Guinea pigs at first parturition by multiplex PCR
Original languageSpanish
Pages (from-to)679-686
Number of pages8
JournalRevista de Investigaciones Veterinarias del Peru
Volume28
Issue number3
DOIs
StatePublished - 1 Jul 2017
Externally publishedYes

Bibliographical note

Funding Information:
Los autores expresan su agradecimien- to al Programa Nacional de Innovación para la CompetitividadyProductividad –Innóvate Perú, fuente financiadora del Proyecto «De-sarrollo de una vacuna para el control y pre-vención de la salmonelosis en la producción de cuyes», Contrato N° 362-PNICP-PIAP-2014.Alos propietarios del centro de crianza de cuyes, Sres. Eduardo López yYenny Castro, por brindar lasfacilidades para realizar el estudio. A Laura Córdova, Gerardo Díaz y Geraldine Marcelo por su apoyo incondicio-nal durante las tomas de muestra y a Rocío

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