Identificación molecular de Salmonella Typhimurium en cuyes al primer parto mediante la técnica de PCR múltiple

Translated title of the contribution: Molecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR

O. Ana Chero, A. Raúl Rosadio, M. Geraldine Marcelo, O. Gerardo Díaz, A. Ronald Jiménez, G. Yenny Castro, H. Lenin Maturrano

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

Translated title of the contributionMolecular identification of Salmonella Typhimurium and Enteritidis in Guinea pigs at first parturition by multiplex PCR
Original languageSpanish
Pages (from-to)679-686
Number of pages8
JournalRevista de Investigaciones Veterinarias del Peru
Volume28
Issue number3
DOIs
StatePublished - 11 Oct 2017

Bibliographical note

Funding Information:
Los autores expresan su agradecimiento al Programa Nacional de Innovaci?n para la Competitividad y Productividad - Inn?vate Per?, fuente financiadora del Proyecto ?Desarrollo de una vacuna para el control y prevenci?n de la salmonelosis en la producci?n de cuyes?, Contrato N? 362-PNICP-PIAP-2014. A los propietarios del centro de crianza de cuyes, Sres. Eduardo L?pez y Yenny Castro, por brindar las facilidades para realizar el estudio. A Laura C?rdova, Gerardo D?az y Geraldine Marcelo por su apoyo incondicional durante las tomas de muestra y a Roc?o Rimac por su aporte en el desarrollo del estudio.

Publisher Copyright:
© 2017 Universidad Nacional Mayor de San Marcos. All rights reserved.

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