Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

Sheila Akinyi; Tonya Hayden; Dionicia Gamboa; Katherine Torres; Jorge Bendezu; Joseph F. Abdallah; Sean M. Griffing; Wilmer Marquiño Quezada; Nancy Arrospide; Alexandre Mac Edo De Oliveira; Carmen Lucas; Alan J. Magill; David J. Bacon; John W. Barnwell; Venkatachalam Udhayakumar

doi:10.1038/srep02797

Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

Sheila Akinyi, Tonya Hayden, Dionicia Gamboa, Katherine Torres, Jorge Bendezu, Joseph F. Abdallah, Sean M. Griffing, Wilmer Marquiño Quezada, Nancy Arrospide, Alexandre Mac Edo De Oliveira, Carmen Lucas, Alan J. Magill, David J. Bacon, John W. Barnwell, Venkatachalam Udhayakumar

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Abstract

The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.

Original language	English
Article number	2797
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep02797
State	Published - 2013

Bibliographical note

Funding Information:
We wish to thank all members of the study team who originally contributed to the collection of samples and study participants for their permission to use these samples. We acknowledge funding support from the Amazon Malaria Initiative which is supported by the U.S. Agency for International Development. S.A. and T.H. were supported by the American Society of Microbiology/CDC Postdoctoral Fellowship Program. D.G. is supported by DGCD (Framework Agreement 03, 2008–2013, Project 95502); D.G. and J.B. are supported by the Peru/Brazil International Center of Excellence in Malaria Research U19AI089681 (NIH/NIAID, United States Public Health Service, USA). K.T. is supported by the NIH/Fogarty International Center Global Infectious Diseases Training Grant D43TW007120 (IMTAvH-UCSD). A.M.O., C.L., J.W.B. and V.U. are employees of the U.S. Government and D.J.B. is a military service member. This work was prepared as part of their official duties. We thank Dr. Fredrik Vannberg of the Georgia Institute of Technology and Mr. Ira F. Goldman of the Centers for Disease Control and Prevention for critical review of the manuscript.

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Akinyi, S., Hayden, T., Gamboa, D., Torres, K., Bendezu, J., Abdallah, J. F., Griffing, S. M., Quezada, W. M., Arrospide, N., De Oliveira, A. M. E., Lucas, C., Magill, A. J., Bacon, D. J., Barnwell, J. W., & Udhayakumar, V. (2013). Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru. Scientific Reports, 3, Article 2797. https://doi.org/10.1038/srep02797

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title = "Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru",

abstract = "The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.",

author = "Sheila Akinyi and Tonya Hayden and Dionicia Gamboa and Katherine Torres and Jorge Bendezu and Abdallah, {Joseph F.} and Griffing, {Sean M.} and Quezada, {Wilmer Marqui{\~n}o} and Nancy Arrospide and {De Oliveira}, {Alexandre Mac Edo} and Carmen Lucas and Magill, {Alan J.} and Bacon, {David J.} and Barnwell, {John W.} and Venkatachalam Udhayakumar",

note = "Funding Information: We wish to thank all members of the study team who originally contributed to the collection of samples and study participants for their permission to use these samples. We acknowledge funding support from the Amazon Malaria Initiative which is supported by the U.S. Agency for International Development. S.A. and T.H. were supported by the American Society of Microbiology/CDC Postdoctoral Fellowship Program. D.G. is supported by DGCD (Framework Agreement 03, 2008–2013, Project 95502); D.G. and J.B. are supported by the Peru/Brazil International Center of Excellence in Malaria Research U19AI089681 (NIH/NIAID, United States Public Health Service, USA). K.T. is supported by the NIH/Fogarty International Center Global Infectious Diseases Training Grant D43TW007120 (IMTAvH-UCSD). A.M.O., C.L., J.W.B. and V.U. are employees of the U.S. Government and D.J.B. is a military service member. This work was prepared as part of their official duties. We thank Dr. Fredrik Vannberg of the Georgia Institute of Technology and Mr. Ira F. Goldman of the Centers for Disease Control and Prevention for critical review of the manuscript.",

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Akinyi, S, Hayden, T, Gamboa, D, Torres, K, Bendezu, J, Abdallah, JF, Griffing, SM, Quezada, WM, Arrospide, N, De Oliveira, AME, Lucas, C, Magill, AJ, Bacon, DJ, Barnwell, JW & Udhayakumar, V 2013, 'Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru', Scientific Reports, vol. 3, 2797. https://doi.org/10.1038/srep02797

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T1 - Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

AU - Akinyi, Sheila

AU - Hayden, Tonya

AU - Gamboa, Dionicia

AU - Torres, Katherine

AU - Bendezu, Jorge

AU - Abdallah, Joseph F.

AU - Griffing, Sean M.

AU - Quezada, Wilmer Marquiño

AU - Arrospide, Nancy

AU - De Oliveira, Alexandre Mac Edo

AU - Lucas, Carmen

AU - Magill, Alan J.

AU - Bacon, David J.

AU - Barnwell, John W.

AU - Udhayakumar, Venkatachalam

N1 - Funding Information: We wish to thank all members of the study team who originally contributed to the collection of samples and study participants for their permission to use these samples. We acknowledge funding support from the Amazon Malaria Initiative which is supported by the U.S. Agency for International Development. S.A. and T.H. were supported by the American Society of Microbiology/CDC Postdoctoral Fellowship Program. D.G. is supported by DGCD (Framework Agreement 03, 2008–2013, Project 95502); D.G. and J.B. are supported by the Peru/Brazil International Center of Excellence in Malaria Research U19AI089681 (NIH/NIAID, United States Public Health Service, USA). K.T. is supported by the NIH/Fogarty International Center Global Infectious Diseases Training Grant D43TW007120 (IMTAvH-UCSD). A.M.O., C.L., J.W.B. and V.U. are employees of the U.S. Government and D.J.B. is a military service member. This work was prepared as part of their official duties. We thank Dr. Fredrik Vannberg of the Georgia Institute of Technology and Mr. Ira F. Goldman of the Centers for Disease Control and Prevention for critical review of the manuscript.

PY - 2013

Y1 - 2013

N2 - The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.

AB - The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.

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U2 - 10.1038/srep02797

DO - 10.1038/srep02797

M3 - Artículo

C2 - 24077522

AN - SCOPUS:84885092153

SN - 2045-2322

VL - 3

JO - Scientific Reports

JF - Scientific Reports

M1 - 2797

ER -

Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

Abstract

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