Alelos mutantes asociados a la resistencia a cloroquina y sulfadoxina-pirimetamina en Plasmodium falciparum de las fronteras Ecuador-Perú y Ecuador-Colombia

Translated title of the contribution: Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders

Nancy Arrospide Velasco, Gisely Hijar-Guerra, Doménica de Mora, César Eduardo Diaz-Cortéz, Raúl Veloz-Perez, Sonia Gutierrez, Cesar Augusto Cabezas Sanchez

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The frequency of mutations in pfCRT and DHFR/DHPS genes of Plasmodium falciparum associated with resistance to chloroquine and sulfadoxine-pyrimethamine was evaluated in 83 strains from the districts of Esmeralda and Machala, located on the borders of Ecuador-Peru and Ecuador-Colombia in 2002. Polymerase chain reaction (PCR), conventional and its variants, was used. Mutations in the pfCRT gene were found in more than 90% of the samples from Esmeralda and Machala. For the DHFR gene, 90% of the strains were mutant samples from Esmeralda, 3 were double mutations and 1 was a triple mutation. In Machala, 25% were simple mutant forms and 75% mixed mutant forms (wild forms/mutant). In conclusion, resistance to chloroquine has been fixed in strains carrying K76T pfCRT mutation, whereas genetic imprinting for resistance to pyrimethamine is evolving, particularly in the district of Esmeralda.

Translated title of the contributionMutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders
Original languageSpanish
Pages (from-to)282-287
Number of pages6
JournalRevista Peruana de Medicina de Experimental y Salud Publica
Volume31
Issue number2
StatePublished - 2014
Externally publishedYes

Fingerprint Dive into the research topics of 'Mutant alleles associated to chloroquine and sulfadoxine-pyrimethanime resistance in Plasmodium falciparum of the Ecuador-Peru and Ecuador-Colombia borders'. Together they form a unique fingerprint.

Cite this