Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50 ) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 μg/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 μL/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice.
|Translated title of the contribution||Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama)|
|Number of pages||8|
|Journal||Revista Peruana de Medicina Experimental y Salud Publica|
|State||Published - 1 Jul 2020|
Bibliographical noteFunding Information:
Fuentes de financiamiento: El presente artículo deriva de un estudio más amplio financiado por el Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica (FONDECYT, Perú) Convenio N.o 188-2015-FONDECYT, además del financiamiento no monetario del INS.
© 2020, Instituto Nacional de Salud. All rights reserved.
- Bothrops atrox
- Immune Sera
- Lama glama
- Lethal Dose 50
- Mortality (Source: MeSH NLM)