Neutralización de la actividad letal del veneno de serpiente bothrops atrox por suero hiperinmune de llama (Lama glama)

Translated title of the contribution: Neutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama)

Henri Bailon Calderon, Elizabeth Gaby Colque Alave, Verónica Olga Yaniro Coronel, Carlos Padilla Rojas, Marco Galarza Pérez, Omar Alberto Cáceres Rey, César Bonilla Ferreyra, Benigno Tintaya Felix, David García Neyra, Rosalina Rosio Inga Arellano, Silvia Seraylan Ormachea, Harrison Montejo Arevalo

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To evaluate the capacity of the hyperimmune llama serum (Lama glama) to neutralize the lethal activity of Bothrops atrox venom in laboratory mice. Materials and methods: Mean lethal dose (LD50 ) was calculated from a Bothrops atrox venom sample pool from Peru. The antibody titers were measured by ELISA assay; and the immune serum neutralization potency was measured by calculating the mean effective dose (ED50) during the immunization period. Results: The venom’s LD50 was 3.96 μg/g; similar to what was found in other studies about Bothrops atrox carried out in Peru. The titers of antibodies against the venom increased rapidly in the llama, demonstrating a fast immune response; however, the neutralization capacity increased slowly and required several doses and immunization reinforcements, obtaining a ED50 of 3.30 μL/g mouse and a neutralization potency of 3.6 mg/mL after 15 immunizations. Conclusions: The hyperimmune llama serum is able to neutralize the lethality of the Bothrops atrox venom from Peru in laboratory mice.

Translated title of the contributionNeutralization of the lethal activity from bothrops atrox venom by hyperimune llama serum (Lama glama)
Original languageSpanish
Pages (from-to)446-453
Number of pages8
JournalRevista Peruana de Medicina de Experimental y Salud Publica
Volume37
Issue number3
DOIs
StatePublished - 1 Jul 2020
Externally publishedYes

Bibliographical note

Funding Information:
Fuentes de financiamiento: El presente art?culo deriva de un estudio m?s amplio financiado por el Fondo Nacional de Desarrollo Cient?fico, Tecnol?gico y de Innovaci?n Tecnol?gica (FONDECYT, Per?) Convenio N.o 188-2015-FONDECYT, adem?s del financiamiento no monetario del INS.

Publisher Copyright:
© 2020, Instituto Nacional de Salud. All rights reserved.

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

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