Oxidative balance in follicular fluid of art patients of advanced maternal age and blastocyst formation

Ricardo Pella, Silvia Suárez-Cunza, Patricia Orihuela, Francisco Escudero, Ygor Pérez, Mario García, Ingrid Zorrilla, Paola Berrío, Sergio Romero

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objective: To evaluate the follicular fluid oxidative balance of infertile patients of advanced-maternal-age and the correlation between oxidative imbalance in the follicu-lar fluid and the embryological outcomes. Methods: Follicular fluid (FF) from infertile patients of advanced-maternal-age undergoing ART treatments were collected and frozen in cryovials at-86°C until further use, and analyzed at the Biochemistry and Nutrition Institute of San Marcos University. As controls, we used FF from oocyte donors. The FF was then assayed for oxidative balance by ABTS, FRAP and TBARS assays. In order to establish the correlation between oxidative balance and embryo quality, we correlated the number of usable blastocysts (freshly transferred or frozen blastocysts) with the results from ABTS, FRAP and TBARS. Results: Follicular fluid from patients of Advanced-ma-ternal-age (AMA group) significantly differed from the val-ues found in the control group; the ABTS value was higher and the FRAP value was lower, in comparison to the FF from oocyte donors (control group). The lipid peroxidation was not different between those two groups. Furthermore, there was no significant correlation among the results of the assays, or when correlated with the proportion of usable blastocysts. Conclusion: Ovarian oxidative balance seems to be critical for oocyte quality in advanced-maternal-age pa-tients; however, we still need more studies on oxidative stress indicators, on a larger set of patients.

Original languageEnglish
Pages (from-to)296-301
Number of pages6
JournalJornal Brasileiro de Reproducao Assistida
Issue number3
StatePublished - 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020, Sociedade Brasileira de Reproducao Assistida. All rights reserved.


  • Chromosome alignment
  • IVM
  • Oocyte
  • Oxidative stress


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