Paneth cell identification in the small intestine of guinea pig offsprings (Cavia porcellus)

Maria Elith Vasquez Cachay, Erika Pebe Gomez, Jose Luis Rodriguez Gutierrez, Boris Antonio Lira Mejia, Néstor Falcón Pérez, Carolina Natalia Zanuzzi, Claudio Barbeito

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The aim of this study was to determine the presence, number, and morphometrical characteristics of Paneth cells (PC) in the small intestine of guinea pigs during lactation. We used 48 pups from 0 to 15 days old. Samples from small intestine were fixed in 10% buffered formaldehyde (pH 7.4) and processed for histological and morphometrical studies using hematoxylin and eosin (HE), Phloxine tartrazine or Masson's Trichome staining, or immunohistochemistry for lysozyme. PC were morphologically identified at day 2 using Masson's Trichome or Phloxine tartrazine stainings, and at day 4 using HE, whereas using immunohistochemistry they were recognized from birth. Morphometrical differences were found between the intestinal sections at each age studied, and within each section during the first weeks of life. In all developmental stage, the highest number of PC was observed in the duodenum of 13 days old guinea pigs. Our results confirm the presence of PC in the small intestine of guinea pigs from birth.

Original languageEnglish
Pages (from-to)856-863
Number of pages8
JournalAnatomical Record
Volume297
Issue number5
DOIs
StatePublished - May 2014
Externally publishedYes

Keywords

  • Guinea pig
  • Immunohistochemistry
  • Lactation
  • Lysozyme
  • Paneth cells

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