This work used eleven Peruvian snake venoms (Bothrops andianus, Bothrops atrox, Bothrops barnetti, Bothrops castelnaudi, Bothriopsis chloromelas, Bothrocophias microphthalmus, Bothrops neuwiedi, Bothriopsis oligolepis, Bothriopsis peruviana, Bothrops pictus and Bothriopsis taeniata) to perform in vitro experimentation and determine its main characteristics. Hyaluronidase (HYAL), phospholipase A2 (PLA2), snake venom metalloproteinase (SVMP), snake venom serine protease (SVSP) and l-amino acid oxidase (LAAO) activities; toxicity by cell viability assays using MGSO3, VERO and HeLa cell lineages; and crossed immunoreactivity with Peruvian (PAV) and Brazilian (BAV) antibothropic polyvalent antivenoms, through ELISA and Western Blotting assays, were determined. Results show that the activities tested in this study were not similar amongst the venoms and each species present their own peculiarities, highlighting the diversity within Bothrops complex. All venoms were capable of reducing cell viability of all tested lineages. It was also demonstrated the crossed recognition of all tested venoms by both antivenoms.
Bibliographical noteFunding Information:
This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico , Brazil (CNPq) (Chamada Bilateral nº 17/2013 , processo: 490269/2013-3), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior , Brazil - CAPES (TOXINOLOGIA N o 23038000825/2011-63 ) and by Fundação de Amparo a Pesquisa do Estado de Minas Gerais , Brazil (FAPEMIG).
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- In vitro assays
- Peruvian snakes
- Venom characterization