Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.

Jorge León, Guillermina Tapia, Rita Avalos

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1 Scopus citations

Abstract

© 2005 Facultad de Ciencias Biológicas UNMSM. A member of the genus Alteromonas sp. (strain N22.C) isolated from marine neuston and substance-producer which inhibit growth of ichthyopathogenics was characterized phenotipically by means of morphological, physiological and biochemical conventional tests. To determine the chemical nature of the inhibitory substance, raw extracts of supernatans from cultured strains were precipitated with increasing concentrations of Ammonium Sulphate up to 70% and filtered through columns of Sephadex G-25. Assays by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) revealed that the antimicrobial substance is a proteinaceous compound with a molecular mass of approximately 34000 Da, which lacks sugar associated residues of it. Other assays made with raw semipurified fractions extracts of Alteromonas sp N22.C show the wide spectrum of antibiotic activity against pathogenic bacterial strains of fish, mollusks and crustaceans.
Original languageAmerican English
Pages (from-to)359-368
Number of pages10
JournalRevista Peruana de Biologia
StatePublished - 1 Jan 2005

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